NCS1 Antikörper (Intracellular)

Produktnummer ABIN7043367

Produktdetails anti-NCS1 Antikörper

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Bindungsspezifität: AA 40-53, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NCS1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Neuronal Calcium Sensor 1

Spezifität: Intracellular

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-NCS1 Antibody is directed against an epitope of the rat Neuronal calcium sensor 1. Anti-NCS1 Antibody (ABIN7043367, ABIN7044204 and ABIN7044205) can be used in western blot analysis. It has been designed to recognize NCS1 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)SGQLDAAGFQKIYK, corresponding to amino acid residues 40-53 of rat NCS1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200-1:400

Kommentare:

Negative Control: (ABIN7236071)

Blocking Peptide: (ABIN7236071)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu NCS1

Target: NCS1 (Neuronal Calcium Sensor 1 (NCS1))

Andere Bezeichnung: NCS1 (NCS1 Produkte)

Hintergrund: Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP,Neuronal calcium sensor-1 (NCS-1) is a member of the neuronal calcium sensor family of EF-hand Ca2+-binding proteins that triggers different biological processes to regulate signal transduction in neurons and photoreceptor cells. This family of proteins includes 15 members and is primarily expressed in neurons. The protein interacts with the G-protein coupled receptor (GPCR) Dopamine D2 receptor (D2R), and the cognate kinases GRK1 and GRK2. NCS-1 responds to changes in intracellular Ca2+ concentration and is involved in many important physiological functions including the regulation of neurotransmitter release, synaptic plasticity, learning and memory, neurite growth, and neuronal survival1,2.NCS-1 structure is characterized by 4 helix-loop-helix motifs, called EF-hand motifs able to bind Ca2+ ions. These domains are separated by a short loop containing a conserved glycine residue. NCS-1 is targeted to the plasma membrane by an N-terminal myristoylation group through the action of N-miristoyltransferase. Opposite to the EF-motifs is a wide hydrophobic crevice (HC). This area has the ability to interact with various physiological targets2,3.NCS-1 dysfunctions are linked to mental conditions, such as bipolar disorder, schizophrenia and autism2.

Alternative names: NCS1, Neuronal calcium sensor 1, Frequenin homolog, Frequenin-like protein, FREQ, FLUP

Gen-ID: 65153

NCBI Accession: NM_014286

UniProt: P62168