Neuroligin 3 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886634

Der Kaninchen Polyklonal anti-Neuroligin 3 Antikörper wird verwendet zum Nachweis von Neuroligin 3 in Proben von Ratte. Er wurde validiert für WB und IHC.

Kurzübersicht für Neuroligin 3 Antikörper (Extracellular, N-Term) (ABIN7886634)

Target: Neuroligin 3 (NLGN3)

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Neuroligin 3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Neuroligin 3 Antikörper

Bindungsspezifität: AA 656-671, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to Neuroligin 3

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-Neuroligin 3 (extracellular) Antibody (ABIN7043363, ABIN7044684 and ABIN7044685) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, analysis. The antibody recognizes an extracellular epitope, and could potentially be used for detecting the protein in living cells. It has been designed to recognize NLGN3 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)SHITRRPNGKTWSTKR, corresponding to amino acid residues 656-671 of rat NLGN3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7236056)

Blocking Peptide: (ABIN7236056)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Neuroligin 3

Target: Neuroligin 3 (NLGN3)

Andere Bezeichnung: NLGN3

Hintergrund:

Synonyms: NLGN3, Gliotactin homolog, HNL3, NL3

Description: Neuroligins (Nlgns) are a family of postsynaptic transmembrane cell adhesion proteins that play a crucial role in excitation and inhibition balance through their regulation of GABAergic and glutamatergic signaling. 5 Neuroligin family members have been identified in humans: NL1, NL2, NL3, NL4X, and NL4Y. NL 1-3 have close homologs in mice. Neurogilins have been identified in rodents, chicken, Drosophila, and C. elegans. Neuroligin structure contains extracellular acetylcholinesterase like domain that lacks esterase activity, a single transmembrane domain, and a short cytoplasmic tail containing a type I PDZ-domain-binding motif that functions in intracellular protein-protein interactions and signaling processes. Neuroligins and neurexins are known to interact with each other1,2.Neuroligin 3 is encoded by the Nlgn3 gene and seems to be ubiquitously expressed in the brain. Despite this fact, Neuroligin 3 functions in synaptic transmission appears to be region specific, affecting the somatosensory cortex and the hippocampus differently2.In humans, a missense mutation in Nlgn3 (Nlgn3R451C) causing reduced synaptic expression of the protein is associated with non-syndromic autism spectrum disorders. In addition, Nlgn3 deletion shows impaired social interaction and vocal communication in rodent models.

Gen-ID: 171297

NCBI Accession: NM_018977

UniProt: Q62889

Pathways: Synaptic Membrane