Neuroligin 1 Antikörper (Extracellular)

Produktnummer ABIN7043361

Produktdetails anti-Neuroligin 1 Antikörper

Target: Neuroligin 1 (NLGN1)

Bindungsspezifität: AA 648-661, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Neuroligin 1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Neuroligin 1

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 13,14 amino acid residues identical

Produktmerkmale: Anti-Neuroligin 1 (extracellular) Antibody is directed against an extracellular epitope of rat Neuroligin 1. Anti-Neuroligin 1 (extracellular) Antibody (ABIN7043361, ABIN7044680 and ABIN7044681)) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize NLGN1 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KVPSTDITLRPTRK, corresponding to amino acid residues 648-661 of rat NLGN1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:800

Kommentare:

Negative Control: BLP-NR035

Blocking Peptide: BLP-NR035

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Neuroligin 1

Target: Neuroligin 1 (NLGN1)

Andere Bezeichnung: NLGN1 (NLGN1 Produkte)

Hintergrund: NLGN1, NL1,The NLGN1 gene encodes a member of a family of neuronal cell surface proteins. Neuroligins act as ligands for β-neurexins, cell adhesion proteins located presynaptically. Neuroligin and β-neurexin "shake hands," resulting in the connection between the two neurons and the production of a synapse. Neuroligin 1 may be involved in the formation and remodeling of central nervous system synapses1.Proteins of this family may also mediate signaling by recruiting and stabilizing key synaptic components. This is done by interacting with other postsynaptic proteins to localize neurotransmitter receptors and channels in the postsynaptic density as the cell matures1.Mutations in the neuroligin genes are implicated in autism and other cognitive disorders2.Neuroligins have been found to play a role in angiogenesis, specifically in human pheripheral tissues3.Neuroligins bind with the help of Ca2+ to the α-neurexin LNS (laminin, neurexin and sex hormone-binding globulin-like folding units) domains and to the β-neurexin LNS domain which then establishes a heterophilic trans-synaptic recognition code4. This binding results in a heterotetramer made up of a neuroligin-1 dimer and two neurexin-1 β monomers5.

Alternative names: Neuroligin 1, NLGN1, NL1

Gen-ID: 116647

NCBI Accession: NM_014932

UniProt: Q62765

Pathways: Synaptic Membrane, Synaptic Vesicle Exocytosis