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MANF Antikörper (Mature)

Der Kaninchen Polyklonal Anti-MANF-Antikörper wurde für WB, IHC und IF validiert. Er ist geeignet, MANF in Proben von Human zu detektieren.
Produktnummer ABIN7043330

Kurzübersicht für MANF Antikörper (Mature) (ABIN7043330)

Target

Alle MANF Antikörper anzeigen
MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

Reaktivität

  • 56
  • 19
  • 17
  • 2
  • 2
  • 2
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
Human

Wirt

  • 36
  • 19
  • 1
Kaninchen

Klonalität

  • 39
  • 17
Polyklonal

Konjugat

  • 42
  • 2
  • 2
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Dieser MANF Antikörper ist unkonjugiert

Applikation

  • 48
  • 21
  • 20
  • 16
  • 12
  • 6
  • 5
  • 4
  • 1
Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
  • Bindungsspezifität

    • 10
    • 7
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    AA 91-104, Mature

    Verwendungszweck

    A Rabbit Polyclonal Antibody to MANF

    Spezifität

    Mature MANF protein

    Kreuzreaktivität

    Human, Maus, Ratte

    Homologie

    Rat,mouse - identical

    Produktmerkmale

    Anti-MANF/ARMET Antibody (ABIN7043330, ABIN7044787 and ABIN7044788) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize MANF from human, rat and mouse samples.

    Aufreinigung

    Affinity purified on immobilized antigen.

    Immunogen

    Immunogen: Synthetic peptide

    Immunogen Sequence: (C)EVSKPLAHHIPVEK, corresponding to amino acid residues 91-104 of human MANF

    Isotyp

    IgG
  • Applikationshinweise

    Antigen preadsorption control: 1 μg peptide per 1 μg antibody

    Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:600

    Application Dilutions Western blot wb: 1:200

    Kommentare

    Cited Application: IHC

    Negative Control: (ABIN7235952)

    Blocking Peptide: (ABIN7235952)

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Format

    Lyophilized

    Rekonstitution

    Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

    Konzentration

    1 mg/mL

    Buffer

    PBS pH 7.4

    Lagerung

    4 °C,-20 °C

    Informationen zur Lagerung

    Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

    Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

  • Target

    MANF (Mesencephalic Astrocyte-Derived Neurotrophic Factor (MANF))

    Andere Bezeichnung

    MANF

    Hintergrund

    Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP,The novel protein mesencephalic astrocyte-derived neurotrophic factor (MANF) is a survival molecule (alternatively referred to as arginine-rich, mutated in early stage tumors, or ARMET) that has high selectivity for dopaminergic neurons of the midbrain1,2. There, it induces proliferative and protective effects against neurodegeneration (usually caused by 6-hydroxydopamine), its therapeutic potential in dopamine-related neurodegenerative disorders such as Parkinson's disease is therefore promising3,4.MANF is also expressed cardiac tissues. As was recently suggested, it induces cardioprotection in the heart and anti-hypertrophic effects via reducing endoplasmic reticulum stress in ischemic conditions in both the heart and the brain. Thus, its effects stretch beyond rescue and protection of dopaminergic neurons alone1,5.Generally, neurotrophic factors (NTFs) provide support, protection, and regulation for neurons - with each group of neurons having their own unique NTFs - both during development and at adulthood. Other noticeable NTFs include neurotrophin-3 and -4 (NT3 and NT4), nerve growth factor (NGF), brain derived neurotrophic factor (BDNF), and glial cell derived neurotrophic factor (GDNF)6.MANF is considered to be evolutionary conserved within Mammalia and beyond (including distant species such as nematode and fruit fly - the latter shares more than 50 % identity with human MANF gene) and is highly mutative in many tumors. It is mostly composed of alpha helices and random coils, its structure essentially differs from any other neurotrophic factor. It is suggested that the protein initiates intracellular signaling cascade by binding to cell-surface lipids and membranes via its cysteine-rich N-terminal. Together with its C-terminal which contains a disulfide bridge that endows a cytoprotective response against ER stress, MANF is implied to be bi-functional3,7.

    Alternative names: MANF/ARMET, Mesencephalic astrocyte-derived neurotrophic factor, Arginine-rich mutated in early stage tumors, ARP

    Gen-ID

    7873

    NCBI Accession

    NM_006010

    UniProt

    P55145
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