EDG4 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886539

Der Kaninchen Polyklonal anti-EDG4 Antikörper wird verwendet zum Nachweis von EDG4 in Proben von Maus. Er wurde validiert für WB, FACS und LCI.

Kurzübersicht für EDG4 Antikörper (Extracellular, N-Term) (ABIN7886539)

Target: EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EDG4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-EDG4 Antikörper

Bindungsspezifität: AA 16-29, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to LPA Receptor 2 (EDG4)

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - identical, human - 13,14 amino acid residues identical

Produktmerkmale: Anti-LPAR2 (EDG4) (extracellular) Antibody (ABIN7043312, ABIN7044568 and ABIN7044569) is a highly specific antibody directed against an epitope of the mouse LPA2 receptor. The antibody can be used in western blot analysis. It recognizes an extracellular epitope and thus is ideal for detecting the receptor in living cells. It has been designed to recognize LPA2 receptor from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)NSGKELSLHWRPKD, corresponding to amino acid residues 16-29 of mouse LPA2 receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235902)

Blocking Peptide: (ABIN7235902)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu EDG4

Target: EDG4 (LPAR2) (Lysophosphatidic Acid Receptor 2 (LPAR2))

Andere Bezeichnung: LPAR2

Hintergrund:

Synonyms: Lysophosphatidic acid receptor 2, LPA receptor 2, LPA-2

Description: Lysophosphatidic acid (LPA) is a lipid mediator that induces various cellular responses including proliferation, migration and inhibition of apoptosis in numerous types of cells via its specific G protein-coupled receptors. At least 5 LPA G-protein coupled receptors (GPCRs) have been identified so far. LPA mediates its effects predominantly by interacting with members of the endothelial differentiation gene (EDG) subfamily of GPCRs: LPA1/EDG2, LPA2/EDG4, and LPA3/EDG71. LPA2 couples to three distinct families of heterotrimeric GTP binding proteins, including Gαq, Gαi, and Gα12/132.LPA2 spans the plasma membrane seven times and contains three extracellular loops and three intracellular loops. LPA2 is unique from the other LPA receptors as it contains two distinct protein-protein interaction domains in the carboxyl-terminal tail (aa 296-351). In the proximal region, LPA2 contains a di-leucine motif and several putative palmitoylated cysteine residues. This region is responsible for associating with zinc-finger proteins3.LPA2 mRNA is expressed in a variety of tissues including human testis, leukocytes, prostate, spleen, thymus, pancreas, and bone marrow3.LPA2-null mice do not show any obvious phenotypic aberrations5. On the other hand, LPA2 expression is increased in ovarian and thyroid cancers and inflammation, suggesting that the main function of LPA2 could become evident under pathophysiological conditions6.

Gen-ID: 53978

NCBI Accession: NM_004720

UniProt: Q9JL06