KIDINS220 Antikörper (C-Term, Intracellular)

Produktnummer ABIN7887043

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch KIDINS220 in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für KIDINS220 Antikörper (C-Term, Intracellular) (ABIN7887043)

Target: KIDINS220 (Kinase D-Interacting Substrate, 220kDa (KIDINS220))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser KIDINS220 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-KIDINS220 Antikörper

Bindungsspezifität: AA 1335-1348, C-Term, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Kidins220

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-Kidins220 Antibody (ABIN7043293, ABIN7045160 and ABIN7045161) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Kidins220 from human, mouse, and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)PRHSNLSWQSQTRR, corresponding to amino acid residues 1335-1348 of rat Kidins220

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:1000

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7235829)

Blocking Peptide: (ABIN7235829)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu KIDINS220

Target: KIDINS220 (Kinase D-Interacting Substrate, 220kDa (KIDINS220))

Andere Bezeichnung: KIDINS220

Hintergrund:

Synonyms: Kinase D-interacting substrate of 220 kDa, Ankyrin repeat-rich membrane-spanning protein, ARMS

Description: The Kidins220 (Kinase D-interacting substrate of 220 kDa) or ARMS (Ankyrin repeat-rich membrane-spanning) protein was originally identified as a substrate for protein kinase D (PKD) in neural cells. Kidins220 is a member of the Kidins220/ARMS and PifA (KAP) family of P-loop nucleotide phosphatases (NTPases).Kidins220 is an integral membrane protein, containing four transmembrane segments in the central part of the molecule, and N- and C-terminal tails both exposed to the cytoplasm. Kidins220 possesses numerous domains that mediate protein-protein interactions. The N-terminus of Kidins220 contains eleven ankyrin repeats which form a concave binding surface that is accessible to molecular partners. Kidins220 contains Walker A and Walker B (involved in the binding of nucleotides) motifs in its juxta-membrane regions flanking the N- and C-termini. The C-terminus of Kidins220 includes several protein-protein interaction domains that are involved in inter- and intra-molecular interactions. The last four amino acids of Kidins220 constitute a PDZ-binding motif that binds the PDZ domain of α-syntrophin, thereby forming a complex that is associated with the EphA4 receptor at the neuromuscular junction1.Neurotrophin signaling is responsible for a wide variety of neuronal functions and is dependent upon two different transmembrane receptor types, the Trk receptor tyrosine kinase and the p75 neurotrophin receptor that belongs to the TNF receptor family. Kidins220 interacts with TrkA, TrkB, TrkC receptors, and with p75NTR. Kidinis220 is rapidly tyrosine phosphorylated after binding of neurotrophins to Trk receptors and provides a docking site for the CrkL-C3G complex, resulting in Rap1-dependent sustained ERK activation2.Kidins220 also associates with NMDA receptors. Upon overstimulation of the NMDA receptor, activity-dependent Ca2+ influx through NMDA receptors leads to a decrease in Kidins220 levels owing to transcriptional downregulation and protein cleavage by calpain1.

Gen-ID: 116478

NCBI Accession: NM_020738

UniProt: Q9EQG6