HCRTR1 Antikörper (3rd Intracellular Loop)

Produktnummer ABIN7886825

Der Kaninchen Polyklonal anti-HCRTR1 Antikörper wird verwendet zum Nachweis von HCRTR1 in Proben von Ratte. Er wurde validiert für WB, IHC, IF und IC.

Kurzübersicht für HCRTR1 Antikörper (3rd Intracellular Loop) (ABIN7886825)

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HCRTR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-HCRTR1 Antikörper

Bindungsspezifität: 3rd Intracellular Loop, AA 256-269

Verwendungszweck: A Rabbit Polyclonal Antibody to Orexin Receptor 1

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 9,14 amino acid residues identical

Produktmerkmale: Anti-Orexin Receptor 1 Antibody (ABIN7043285, ABIN7044883 and ABIN7044884) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX1R from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RNWKRPSEQLEAQH, corresponding to amino acid residues 256-269 of rat OX1R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200-1:500

FC: The optimal concentration should be determined by the user

ICC: 1:200

IHC: 1:50-1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7235802)

Blocking Peptide: (ABIN7235802)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu HCRTR1

Target: HCRTR1 (Hypocretin (Orexin) Receptor 1 (HCRTR1))

Andere Bezeichnung: HCRTR1

Hintergrund:

Synonyms: OX1R, Hypocretin receptor type 1, HCRTR1

Description: Orexin Receptor 1 (OX1R) (also known as hypocretin receptor 1) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference), while OX2R binds both ligands with similar affinities.2,3Both OX1R and OX2R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.OX1R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research in the last few years.OX1R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2 It appears to be involved in the regulation of feeding behavior in rodents since an OX1R antagonist is able to inhibit baseline feeding.2,3The orexin system has been shown to be involved in the regulation of sleep and wakefulness states and OX1R knockout mice show defects in the regulation of rapid eye movement (REM) sleep, among other phenotypic alterations.4 In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Gen-ID: 25593

NCBI Accession: NM_001525

UniProt: P56718

Pathways: Feeding Behaviour