HOMER3 Antikörper (Intracellular)

Produktnummer ABIN7043275

Produktdetails anti-HOMER3 Antikörper

Target: HOMER3 (Homer Homolog 3 (Drosophila) (HOMER3))

Bindungsspezifität: AA 155-169, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HOMER3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck: A Rabbit Polyclonal Antibody to Homer3

Spezifität: Intracellular

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human - identical, mouse - 14,15 amino acid residues identical

Produktmerkmale: Anti-Homer3 Antibody (ABIN7043275, ABIN7045172 and ABIN7045173) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize Homer3 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RSQSADAPGPTERER, corresponding to amino acid residues 155 - 169 of rat Homer3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235770)

Blocking Peptide: (ABIN7235770)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu HOMER3

Target: HOMER3 (Homer Homolog 3 (Drosophila) (HOMER3))

Andere Bezeichnung: HOMER3 (HOMER3 Produkte)

Hintergrund: Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3,The HOMER protein family includes Homer1, 2, and 3. These proteins are mainly expressed in the neuronal post-synaptic density (PSD) where they play a role in post-synaptic signaling and receptor trafficking by forming multivalent links with various receptors and PSD scaffolding proteins. The expression level of Homer proteins in non-neural tissues is very low in comparison with that in the nervous system, HOMER3 also expressed in the lung, spleen, kidney and ovary. In the mouse brain HOMER3 is concentrated in the dendritic spines of Purkinje cells connecting the parallel fibers and is also present in their axons, In the hippocampus, HOMER3 is concentrated in the CA2-CA3 regions1,2.All three proteins in the HOMER family have several isoforms as a result of alternative splicing, HOMER3 are shown to exist in both long and short forms, the latter without the C-terminal coiled-coil domains. HOMER3 mRNA is subject to alternative splicing at five different sites giving rise to ten variants of HOMER3 proteins. HOMER1b and HOMER3 have been shown to form a tetramer via the carboxy-terminal domain3.HOMER3 directly binds to type I metabotropic glutamate receptors, which act via phospholipase C to stimulate IP3-mediated release of Ca2+ from intracellular vesicles3.HOMER proteins have also been implicated in axon guidance during brain development4.HOMER3 can regulate transcription and play a very important role in the differentiation and development for some tissues such as muscle and the nervous system. Studies show that HOMER3 abnormal expression plays a role in acute myeloid leukemia (AML). Heterologous expression of HOMER3 in K562 cells inhibits proliferation, influences the cell cycle profile, affects apoptosis and also promotes cell differentiation5.

Alternative names: Homer3, Homer protein homolog 3, Homer scaffold protein 3, VESL3, Homer-3

Gen-ID: 29548

NCBI Accession: NM_001145721

UniProt: Q9Z2X5