GLRB Antikörper (AA 394-405)

Produktnummer ABIN7043260

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GLRB in WB. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für GLRB Antikörper (AA 394-405) (ABIN7043260)

Target: GLRB (Glycine Receptor, beta (GLRB))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLRB Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GLRB Antikörper

Bindungsspezifität: AA 394-405

Verwendungszweck: A Rabbit Polyclonal Antibody to Glycine Receptor β

Spezifität: 2nd intracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-Glycine Receptor beta Antibody is directed against an epitope of the rat glycine receptor β. Anti-Glycine Receptor β Antibody (ABIN7043260, ABIN7044402 and ABIN7044403) can be used in western blot analysis. It has been designed to recognize GlyRβ from human, rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: HISTLQVGETRC, corresponding to amino acid residues 394-405 of rat GlyRbeta

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235724)

Blocking Peptide: (ABIN7235724)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GLRB

Target: GLRB (Glycine Receptor, beta (GLRB))

Andere Bezeichnung: GLRB

Hintergrund: Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRβ,The amino acid glycine mediates neuronal inhibition by activating glycine receptors (GlyRs), which are ligand-gated chloride channels of the nicotinic acetylcholine receptor superfamily. GlyRs are known to mediate postsynaptic inhibition in spinal cord, brain stem and some higher brain regions1. GlyRs are members of the pentameric Cys-loop family of ionotropic neurotransmitter receptors.GlyRs are composed of homologous α (α1-α4) and β subunits. Upon synthesis at the endoplasmic reticulum (ER), all GlyRα subunits can assemble into functional homopentamers that are inserted into the plasma membrane. In contrast, the GlyRβ subunit requires stoichiometric coassembly with GlyRα subunits for ER exit and cell surface expression. Each subunit consists of an extended N-terminal extracellular domain, four transmembrane domains, and a large intracellular loop between transmembrane domains 3 and 4. The β subunit is responsible for synaptic localization of the GlyRs by interacting with the postsynaptic scaffolding protein gephyrin. GlyRβ subunit is reported to interact with the proteins Vacuolar Protein Sorting 35 (Vps35) and Neurobeachin (Nbea)2.GlyRβ mRNA is abundantly found throughout the late embryonic and postnatal brain3.Mutations in GLRB, the gene encoding GlyRβ, have been discovered in patients with hyperekplexia4, in the mouse mutant spastic5 and the zebrafish bandoneon mutant6.

Alternative names: Glycine Receptor beta , Glycine receptor subunit beta, Glycine receptor 58 kDa subunit, GLRB, GlyRbeta

Gen-ID: 25456

NCBI Accession: NM_000824

UniProt: P20781