GLRA1 Antikörper (2nd Cytoplasmic Loop)

Produktnummer ABIN7886409

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GLRA1 in WB, IHC und IF. Es zeigt Reaktivität gegenüber Proben von Ratte.

Kurzübersicht für GLRA1 Antikörper (2nd Cytoplasmic Loop) (ABIN7886409)

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GLRA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-GLRA1 Antikörper

Bindungsspezifität: 2nd Cytoplasmic Loop, AA 350-362

Verwendungszweck: A Rabbit Polyclonal Antibody to Glycine Receptor α1

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 12,13 amino acid residues identical

Produktmerkmale: Anti-Glycine Receptor alpha1 Antibody is directed against an epitope of the rat glycine receptor α1. Anti-Glycine Receptor α1 Antibody (ABIN7043257, ABIN7044394 and ABIN7044395) can be used in western blot and immunohistochemical staining. It has been designed to recognize GlyRα1 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RHHKSPMLNLFQD, corresponding to amino acid residues 350-362 of rat GlyRalpha1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235712)

Blocking Peptide: (ABIN7235712)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GLRA1

Target: GLRA1 (Glycine Receptor, alpha 1 (GLRA1))

Andere Bezeichnung: GLRA1

Hintergrund:

Synonyms: Glycine receptor subunit alpha-1, Glycine receptor 48 kDa subunit, Glycine receptor strychnine-binding subunit, GLRA1, Glyr

Description: Glycine receptors (GlyRs) mediate ionotropic inhibitory neurotransmission in the CNS where they play an essential role in inhibition of motor neurons in the spinal cord and brainstem1. The glycine receptor is composed of three ligand-binding subunits of 48 kDa (α) and two homologous polypeptides of 58 kDa (β) that span the postsynaptic membrane in a pentameric arrangement to form a Cl--selective channel2. So far, at least three different α-subunit variants of the GlyR have been identified: α1, α2 and α33. Homo-oligomeric α2 receptors have a low strychnine-binding affinity and are considered to represent a neonatal form of GlyR, which is replaced during development by receptors containing the α1 subunit. Adult GlyRs are predominantly composed of α1 and β subunits, the α3 subunit being expressed at low levels in only a few brain regions4.GlyRα1 subunits are widely expressed and contribute to many processes in the CNS, including inflammatory pain perception, modulation of auditory and visual pathways, and neurotransmission in the cerebellar cortex5.Mutations in glycine receptor α1 subunits cause human hereditary hyperekplexia, a disorder characterized by an exaggerated startle response, which is a consequence of the role of GlyRs in motor reflex circuits of the spinal cord6.

Gen-ID: 25674

NCBI Accession: NM_000171

UniProt: P07727

Pathways: Synaptic Membrane