Metabotropic Glutamate Receptor 4 Antikörper (Extracellular)

Produktnummer ABIN7043249

Produktdetails anti-Metabotropic Glutamate Receptor 4 Antikörper

Target: Metabotropic Glutamate Receptor 4 (GRM4) (Glutamate Receptor, Metabotropic 4 (GRM4))

Bindungsspezifität: AA 33-44, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Metabotropic Glutamate Receptor 4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to Metabotropic Glutamate Receptor 4

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,human - identical

Produktmerkmale: Anti-mGluR4 (extracellular) Antibody is directed against an extracellular epitope of rat mGluR4. Anti-mGluR4 (extracellular) Antibody (ABIN7043249, ABIN7044344 and ABIN7044345)) can be used in western blot and live cell imaging applications. It has been designed to recognize mGluR4 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: KPKGHPHMNSIR(C), corresponding to amino acid residues 33-44 of rat mGluR4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-GC014

Blocking Peptide: BLP-GC014

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Metabotropic Glutamate Receptor 4

Target: Metabotropic Glutamate Receptor 4 (GRM4) (Glutamate Receptor, Metabotropic 4 (GRM4))

Andere Bezeichnung: GRM4 (GRM4 Produkte)

Hintergrund: Metabotropic glutamate receptor 4, GRM4, mGlu4, GPRC1D,Metabotropic glutamate receptors (mGluRs) belong to the super family of G-protein coupled receptors (seven transmembrane proteins). mGluRs are further divided into subfamilies: group I mGluRs (mGluR1 and mGluR5) which couple to Gq, thereby activating phospholipase C (PLC). Group II which include mGluR2 and mGluR3 couple to Gi, therefore inhibit the formation of adenylate cyclase. mGluR4, 6, 7, 8 which belong to group III also inhibit adenylate cyclase formation by coupling to Gi1.The C-terminus of these receptors has important functions in modulating their activity. This region is important for G-protein coupling, post-translational modifications like phosphorylation as well as protein-protein interactions. The C-terminal region is also subject to alternative splicing2.mGluR4 is predominantly expressed presynaptically in neurons and in the cerebellum. A splice variant of the protein is expressed in taste buds. This variant lacks a large portion of the N-terminus and is normally referred to taste mGluR43,4. It is responsible (along with taste mGluR1) for mediating the taste of monosodium glutamate (or unami)2,4.mGluR4 knock out mice display impaired cerebellar synaptic plasticity as well as some learning disabilities5. In addition, this receptor has emerged as a target for the treatment of Parkinson's disease6.

Alternative names: mGluR4, Metabotropic glutamate receptor 4, GRM4, mGlu4, GPRC1D

Gen-ID: 24417

NCBI Accession: NM_000841

UniProt: P31423