Glucagon Receptor Antikörper (Extracellular)

Produktnummer ABIN7043218

Produktdetails anti-Glucagon Receptor Antikörper

Target: Glucagon Receptor (GCGR)

Bindungsspezifität: AA 200-212, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Glucagon Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck: A Rabbit Polyclonal Antibody to Glucagon Receptor

Spezifität: 1st extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 12,13 amino acid residues identical

Produktmerkmale: Anti-Glucagon Receptor (extracellular) Antibody is directed against an epitope of the rat glucagon receptor. Anti-Glucagon Receptor (extracellular) Antibody (ABIN7043218, ABIN7044410 and ABIN7044411)) can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GCGR from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KTRYSQKIGDDLS, corresponding to amino acid residues 200-212 of rat GCGR

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-GR024

Blocking Peptide: BLP-GR024

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Glucagon Receptor

Target: Glucagon Receptor (GCGR)

Andere Bezeichnung: GCGR (GCGR Produkte)

Hintergrund: GCGR, GLR,The glucagon receptor (GCGR) is one of the 15 members of the secretin-like (class B) family of G-protein-coupled receptors (GPCRs) in humans. Glucagon is a highly conserved 29 amino acid protein processed from proglucagon by PC2 in pancreatic α-cells. The principal actions of glucagon involve regulation of metabolic pathways involved in glucose homeostasis1. Glucagon binding to the GCGR activates adenylyl cyclase through heterotrimeric Gs G proteins, leading to an increase in intracellular cAMP levels and subsequent activation of PKA signaling2. The glucagon receptor is a 485-amino acid protein with a central core consisting of seven membrane-spanning domains, an extracellular N-terminus and a cytoplasmic C-terminal tail2.Glucagon receptors are widely expressed in multiple tissues including the liver, brain, pancreas, heart, kidney, and smooth muscle cell in the gastrointestinal tract and peripheral vasculature1.Abnormal glucagon signaling is associated with hyperglycemia in patients with type 2 diabetes3. Mice with homozygous null mutation of GCGR exhibit chronic hypoglycemia, extreme hyperglucagonemia, and α cell hyperplasia4. GCGR is a potential drug target for type 2 diabetes5.

Alternative names: Glucagon Receptor, GCGR

Gen-ID: 24953

NCBI Accession: NM_000160

UniProt: P30082

Pathways: Carbohydrate Homeostasis, Regulation of Carbohydrate Metabolic Process