GJA4 Antikörper (AA 131-144)

Produktnummer ABIN7043200

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GJA4 in WB. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für GJA4 Antikörper (AA 131-144) (ABIN7043200)

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GJA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-GJA4 Antikörper

Bindungsspezifität: AA 131-144

Verwendungszweck: A Rabbit Polyclonal Antibody to Connexin-37

Spezifität: Intracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 13,14 amino acid residues identical

Produktmerkmale: Anti-Connexin-37 Antibody is directed against an epitope of rat Connexin-37. Anti-Connexin-37 Antibody (ABIN7043200, ABIN7044081 and ABIN7044082) can be used in western blot analysis. It has been designed to recognize Cx37 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)EHQMAKISVAEDGR, corresponding to amino acid residues 131-144 of rat Connexin-37

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:400

Kommentare:

Negative Control: (ABIN7235535)

Blocking Peptide: (ABIN7235535)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GJA4

Target: GJA4 (Gap Junction Protein, alpha 4, 37kDa (GJA4))

Andere Bezeichnung: GJA4

Hintergrund: CX37, Gap junction α4 protein, GJA4,Connexins (Cxs) are a family of transmembrane proteins comprising 21 members in humans that forms gap junction channels (GJCs). Connexins are divided in four groups: α, β, γ and δ. Cx37 is also named α4 since it is the fourth connexin of the alpha group that was identified1,2. Connexins do not function as single proteins, six connexins assemble into connexons (hemi-channels), and two connexons from neighboring cells dock in the extracellular space to form a full gap junction channel. Connexins are responsible for mediating direct communication between adjacent cells by the exchange of ions and small signaling molecules and cytosolic factors1,3. Their structure consists of four transmembrane helices, two extracellular loops, a cytoplasmic loop, a cytoplasmic NH2- and COOH-termini1,2.Connexin-37 is encoded by the GJA4 gene and is expressed in vascular endothelial cells. The protein is responsible for regulating and controlling the growth, proliferation and regeneration of the vascular endothelium by mediating the signal transduction between smooth muscle and endothelial cells. Cx37 knockout mice have a tendency for developing endarterium atheromatous plaques4. Also, a polymorphism in the gene that encodes for Cx37, is associated with increased risk for atherosclerosis and myocardial infarction1.

Alternative names: Connexin-37, CX37, Gap junction alpha4 protein, GJA4

Gen-ID: 25655

NCBI Accession: NM_002060

UniProt: Q03190

Pathways: Cell-Cell Junction Organization