GABRA4 Antikörper (Extracellular)

Produktnummer ABIN7043188

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch GABRA4 in WB, IHC, IF, IC und LCI. Er zeigt eine Reaktivität gegenüber Ratte.

Kurzübersicht für GABRA4 Antikörper (Extracellular) (ABIN7043188)

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GABRA4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC), Live Cell Imaging (LCI)

Produktdetails anti-GABRA4 Antikörper

Bindungsspezifität: AA 37-50, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GABA(A) α4 Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: 14 amino acid residues identical,Mouse, human - 10,rat - 13

Produktmerkmale: Anti-GABA(A) alpha4 Receptor (extracellular) Antibody is directed against an extracellular epitope of rat GABA(A) α4 subunit. Anti-GABA(A) α4 Receptor (extracellular) Antibody (ABIN7043188, ABIN7044305 and ABIN7044306) can be used in western blot, immunohistochemistry and live cell imaging applications. It was designed to recognize GABRA4 from human, rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: NSKDEKLSPENFTR(C), corresponding to amino acid residues 37-50 of rat GABRA4 with replacement of C44 with serine

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: ICC

Negative Control: (ABIN7235496)

Blocking Peptide: (ABIN7235496)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GABRA4

Target: GABRA4 (gamma-aminobutyric Acid (GABA) A Receptor, alpha 4 (GABRA4))

Andere Bezeichnung: GABRA4

Hintergrund: γ-Aminobutyric acid receptor subunit α4, GABRA4,GABA (γ-aminobutyric acid) is the major inhibitory neurotransmitter in the brain. Its production, release, reuptake, and metabolism all occur in the nervous system1.The GABA transmitter interacts with two major types of receptors: ionotropic GABAA receptors (GABAAR) and metabotropic GABAB receptors (GABABR).GABAARs belong to the ligand-gated ion channel superfamily2. GABA inhibits the activity of signal-receiving neurons by interacting with the GABAA receptor on these cells3. Binding of GABA to its GABAA receptor results in conformational changes that open a Cl- channel, producing an increase in membrane conductance that results in inhibition of neural activity2.GABAARs are heteropentamers, in which all five subunits contribute to the pore formation. To date, eight subunit isoforms have been cloned: α, β, γ, δ, ε, π, θ, and ?1. Six α subunit isoforms have been found to exist in mammals (α1-α6). In most cases, native GABAA receptors consist of 2α, 2β, and 1γ subunits. The α subunit is the most common and is expressed ubiquitously. It determines the affinity of GABAARs for allosteric ligands.Each subtype has a unique regional expression in the brain, and individual neurons often express multiple subtypes4. For example, the α4 subunit is detected in the hippocampus, cortex, olfactory bulb and in the basal forebrain5.

Alternative names: GABA(A) α4 Receptor, gamma-Aminobutyric acid receptor subunit alpha4, GABRA4

Gen-ID: 140675

NCBI Accession: NM_000809

UniProt: P28471

Pathways: Synaptic Membrane