GALR2 Antikörper (AA 223-235)

Produktnummer ABIN7043181

Produktdetails anti-GALR2 Antikörper

Target: GALR2 (Galanin Receptor 2 (GALR2))

Bindungsspezifität: AA 223-235

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GALR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck: A Rabbit Polyclonal Antibody to Galanin Receptor Type 2

Spezifität: 3rd intracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - identical, human - 11,13 amino acid residues identical

Produktmerkmale: Anti-GALR2 Antibody is directed against an epitope of the mouse Galanin receptor 2. Anti-GALR2 Antibody (ABIN7043181, ABIN7044398 and ABIN7044399) can be used in western blot and immunohistochemistry applications. It has been designed to recognize GALR2 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GSGSQRAKRKVTR, corresponding to amino acid residues 223-235 of mouse Galanin receptor type 2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235477)

Blocking Peptide: (ABIN7235477)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GALR2

Target: GALR2 (Galanin Receptor 2 (GALR2))

Andere Bezeichnung: GALR2 (GALR2 Produkte)

Hintergrund: Galanin receptor type 2, GALNR2, GAL2R,Galanin is a neuropeptide, consisting of 29-30 amino acid residues, involved in the regulation of cognition, mood, feeding, seizure activity and pain. Galanin exerts its action via three known receptors, the Galanin receptor type 1 (GALR1), Galanin receptor type 2 (GALR2) and Galanin receptor type 3 (GALR3). The Galanin receptors belong to the G-protein coupled receptor superfamily.1-4GALR2 is widely expressed in the brain (with highest levels of mRNA found in the hypothalamus, hippocampus, amygdala and pyriform cortex) and in peripheral tissues such as the small intestine, heart, kidney, and liver. High levels of GALR2 expression were also found in rat dorsal root ganglia.1GALR2 activation increases inositol phosphate hydrolysis, mediates the release of Ca2+ into the cytoplasm from intracellular stores, and opens Ca2+-dependent chloride channels in a PTX-resistant manner, indicating that GALR2 couples to Gq/11 proteins.5

Alternative names: GALR2, Galanin receptor type 2, GALNR2, GAL2R

Gen-ID: 14428

NCBI Accession: NM_003857

UniProt: O88854

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Feeding Behaviour