GPR84 Antikörper (Extracellular)

Produktnummer ABIN7043175

Produktdetails anti-GPR84 Antikörper

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Bindungsspezifität: AA 166-180, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR84 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc))

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR84

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human - 14,15 amino acid residues identical, mouse,rat - 13

Produktmerkmale: Anti-GPR84 (extracellular) Antibody (ABIN7043175, ABIN7044443 and ABIN7044444)) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, immunohistochemistry, and live cell flow cytometry applications. It has been designed to recognize GPR84 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CT(S)SFDRIRGRPYTT, corresponding to amino acid residues 166-180 of human GPR84

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IP

Negative Control: BLP-GR052

Blocking Peptide: BLP-GR052

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR84

Target: GPR84 (G Protein-Coupled Receptor 84 (GPR84))

Andere Bezeichnung: GPR84 (GPR84 Produkte)

Hintergrund: G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33,GPR84 is an orphan G-protein coupled receptor (GPCR) activated by micromolar concentrations of medium-chain fatty acids (MCFAs). MCFAs that activate GPR84 are usually with carbon chain lengths of 9-14, which couples primarily to a pertussis toxin (PTX)-sensitive Gi/o pathway. The most potent agonists of GPR84 includes decanoic acid, undecanoic acid and lauric acid. GPR84 demonstrates an important role in inflammation, immunity, and cancer1-3.GPR84 is predominantly expressed in immune system-related tissues and cells, such as bone marrow, spleen, lung, lymph nodes and brain microglia, T-cells, macrophages and glial cells. Its expression is also detected in adipocytes.Studies have shown that GPR84 activation in murine macrophage-like cell line results in high levels of LPS-stimulated IL-12 p40 production. In addition, GPR84 knockout mice display increased Th2 cytokine production1-3.

Alternative names: GPR84, G-protein coupled receptor 84, Inflammation-related G-protein coupled receptor EX33

Gen-ID: 53831

NCBI Accession: NM_020370

UniProt: Q9NQS5