GPR83 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886443

Der Kaninchen Polyklonal anti-GPR83 Antikörper wird verwendet zum Nachweis von GPR83 in Proben von Maus. Er wurde validiert für WB, IHC, FACS und LCI.

Kurzübersicht für GPR83 Antikörper (Extracellular, N-Term) (ABIN7886443)

Target: GPR83 (G Protein-Coupled Receptor 83 (GPR83))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR83 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR83 Antikörper

Bindungsspezifität: AA 59-71, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR83

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,rat - identical

Produktmerkmale: Anti-GPR83 (extracellular) Antibody (ABIN7043174, ABIN7044445 and ABIN7044446) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR83 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)RRYGAESQNPTVK, corresponding to amino acid residues 59 - 71 of mouse GPR83

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:400

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: 1:3000

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235450)

Blocking Peptide: (ABIN7235450)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR83

Target: GPR83 (G Protein-Coupled Receptor 83 (GPR83))

Andere Bezeichnung: GPR83

Hintergrund:

Synonyms: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR

Description: GPR83 (JP05, GIR, GPR72) is a G-protein coupled receptor (GPCR) highly expressed in the brain, spleen and thymus, suggesting that this receptor may be targeted to treat neurological and immune disorders1.GPCR is a family of receptors responsible for initiation of a myriad of intracellular signaling cascades, these receptors are characterized by an extracellular N-terminus, intracellular C-terminal tail, and seven membrane-spanning α-helices (TM-1 to TM-7)2.In the brain GPR83 is expressed in the hippocampus, amygdala, prefrontal cortex, and in various hypothalamic nuclei where it may play a significant role in learning memory, reward, emotional behaviors, and stress regulation3. GPR83 is also expressed in regulatory T cells (Tregs) and it could potentially be used as a specific marker used to identify natural Tregs4.GPR83 was considered as an "orphan" GPCR. Recently, PEN, a neuropeptide was identified as its endogenous ligand1.

Gen-ID: 14608

NCBI Accession: NM_016540

UniProt: P30731