GPR83 Antikörper (Extracellular)

Produktnummer ABIN7043174

Der Kaninchen Polyklonal Anti-GPR83-Antikörper wurde für WB, IHC, FACS und LCI validiert. Er ist geeignet, GPR83 in Proben von Maus zu detektieren.

Kurzübersicht für GPR83 Antikörper (Extracellular) (ABIN7043174)

Target: GPR83 (G Protein-Coupled Receptor 83 (GPR83))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR83 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR83 Antikörper

Bindungsspezifität: AA 59-71, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR83

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,rat - identical

Produktmerkmale: Anti-GPR83 (extracellular) Antibody (ABIN7043174, ABIN7044445 and ABIN7044446) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize GPR83 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RRYGAESQNPTVK, corresponding to amino acid residues 59 - 71 of mouse GPR83

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:3000

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: (ABIN7235450)

Blocking Peptide: (ABIN7235450)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR83

Target: GPR83 (G Protein-Coupled Receptor 83 (GPR83))

Andere Bezeichnung: GPR83

Hintergrund: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR,GPR83 (JP05, GIR, GPR72) is a G-protein coupled receptor (GPCR) highly expressed in the brain, spleen and thymus, suggesting that this receptor may be targeted to treat neurological and immune disorders1.GPCR is a family of receptors responsible for initiation of a myriad of intracellular signaling cascades, these receptors are characterized by an extracellular N-terminus, intracellular C-terminal tail, and seven membrane-spanning α-helices (TM-1 to TM-7)2.In the brain GPR83 is expressed in the hippocampus, amygdala, prefrontal cortex, and in various hypothalamic nuclei where it may play a significant role in learning memory, reward, emotional behaviors, and stress regulation3. GPR83 is also expressed in regulatory T cells (Tregs) and it could potentially be used as a specific marker used to identify natural Tregs4.GPR83 was considered as an "orphan" GPCR. Recently, PEN, a neuropeptide was identified as its endogenous ligand1.

Alternative names: G-protein coupled receptor 83, G-protein coupled receptor 72, GPR72, Glucocorticoid induced receptor, GIR

Gen-ID: 14608

NCBI Accession: NM_016540

UniProt: P30731