GPR81 Antikörper (C-Term, Intracellular)

Produktnummer ABIN7886507

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch GPR81 in WB und IHC. Es zeigt Reaktivität gegenüber Proben von Maus.

Kurzübersicht für GPR81 Antikörper (C-Term, Intracellular) (ABIN7886507)

Target: GPR81 (G Protein-Coupled Receptor 81 (GPR81))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR81 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-GPR81 Antikörper

Bindungsspezifität: AA 319-332, C-Term, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR81

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): The antibody will not recognize human GPR81 (HCAR1).

Homologie: Rat - 9,14 amino acid residues identical

Produktmerkmale: Anti-GPR81/HCAR1 Antibody (ABIN7043173, ABIN7044514 and ABIN7044515) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. It has been designed to recognize HCAR1 from rat and mouse samples. The antibody will not recognize human GPR81.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CSKSSIDGANRSQR, corresponding to amino acid residues 319-332 of mouse GPR81 (HCAR1)

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:200

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235445)

Blocking Peptide: (ABIN7235445)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR81

Target: GPR81 (G Protein-Coupled Receptor 81 (GPR81))

Andere Bezeichnung: HCAR1

Hintergrund:

Synonyms: Hydroxycarboxylic acid receptor 1, HCA1 Receptor, G-protein coupled receptor 81, GPR81, G Protein-Coupled Receptor 104, LACR1

Description: GPR81 is an orphan G-protein-coupled receptor that belongs to a family of hydroxy-carboxylic acid (HCA) receptors. Its endogenous ligand is L-lactate, a metabolite produced when glycolysis exceeds the capacity to oxidize pyruvate1,2.In addition to being a by-product of metabolism, lactate plays a role in insulin signaling, particularly in insulin mediated anti-lipolytic effects. Likewise, GPR81 is mostly expressed in adipose tissues and its activation results in inhibition of lipolysis and reduced hydrolysis of triglycerides to glycerol and fatty acids, suggesting that GPR81 may be an attractive drug discovery target for metabolic disorders4.Beyond the control of adipocyte lipolysis, GPR81 is also involved in neuronal signaling, regulation of blood flow to the retina. The receptor is overexpressed in several cancer types where it appears to play a role in the adaptation to the ischemic tumor microenvironment5.GPR81, like all other G protein coupled receptors, consists of seven transmembrane domains, in addition it possesses a highly conserved Cys-Glu-Ser-Phe motif in the second extracellular loop, essential for its function and six Cys residues at the extracellular domains that potentially form three disulfide bonds6.

Gen-ID: 243270

NCBI Accession: NM_032554

UniProt: Q8C131