GPR77 Antikörper (Extracellular)

Produktnummer ABIN7043172

Produktdetails anti-GPR77 Antikörper

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Bindungsspezifität: AA 4-18, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR77 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to C5a Anaphylatoxin Receptor 2

Spezifität: Extracellular, N-terminus

Kreuzreaktivität (Details): Will not recognize C5a Anaphylatoxin Chemotactic Receptor 2 from rat or mouse samples.

Homologie: Human only

Produktmerkmale: Anti-Human C5aR2 (extracellular) Antibody (ABIN7043172, ABIN7043928 and ABIN7043929) is a highly specific antibody directed against an epitope of human C5a anaphylatoxin receptor 2 (C5aR2, C5L2). The antibody can be used in western blot analysis. It recognizes an extracellular epitope and is likely to detect the receptor in living cells. It has been designed to recognize C5L2 from human samples only.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DSVSYEYGDYSDLSD, corresponding to amino acid residues 4-18 of human C5a anaphylatoxin chemotactic receptor 2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR77

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Andere Bezeichnung: C5AR2 (GPR77 Produkte)

Hintergrund: C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77,Complement fragment C5a is a pro-inflammatory polypeptide produced during activation of the complement cascade of serum proteins in response to foreign surfaces such as microorganisms and tissue damaged by physical or chemical injury. C5a binds to at least two seven-transmembrane domain receptors, C5aR (C5R1, CD88) and C5L2 (gpr77).Expression of C5L2 (C5a Anaphylatoxin Receptor 2) can be found in various tissues and cells such as astrocytes, neutrophils/macrophages, mast cells, immature dendritic cells, as well as in the brain, lung, heart, kidney, liver, ovary or testis.The C5L2 receptor is a G-protein coupled receptor clustered with other chemoattractant receptors, such as type-II angiotensin-II receptor, bradykinin receptors, the formyl peptide receptor family, ChemR23 and several orphan GPCRs. A small number of single-nucleotide polymorphisms (SNP) have been found in the receptor gene. C5L2 has two synonymous SNPs at 614G/A and 860C, however there are no known associations between these SNPs and human disease.Although C5L2 has the conventional structure of a GPCR, studies have shown that C5L2 does not couple to G proteins owing to the lack of a highly conserved Asp-Arg-Tyr motif, found in the third TM domain1.

Alternative names: C5aR2, C5a anaphylatoxin chemotactic receptor 2, C5a anaphylatoxin receptor 2, Complement component 5a receptor 2, G-protein coupled receptor 77, C5L2, GPR77

Gen-ID: 27202

NCBI Accession: NM_018485

UniProt: Q9P296