GPR55 Antikörper (Extracellular)

Produktnummer ABIN7043163

Dieses Anti-GPR55-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GPR55 in WB und FACS. Geeignet für Human.

Kurzübersicht für GPR55 Antikörper (Extracellular) (ABIN7043163)

Target: GPR55 (G Protein-Coupled Receptor 55 (GPR55))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR55 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-GPR55 Antikörper

Bindungsspezifität: AA 160-174, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR55

Spezifität: 2nd extracellular loop

Kreuzreaktivität (Details): The antibody will not recognize the protein from rat or mouse samples.

Homologie: Human only

Produktmerkmale: Anti-Human GPR55 (extracellular) Antibody (ABIN7043163, ABIN7044200 and ABIN7044201) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and live cell flow cytometry applications. It has been designed to recognize GPR55 from human samples only.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)HGKVEKYMSFHNMSD, corresponding to amino acid residues 160-174 of human GPR55

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200-1:1000

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR55

Target: GPR55 (G Protein-Coupled Receptor 55 (GPR55))

Andere Bezeichnung: GPR55

Hintergrund: G-protein coupled receptor 55,GPR55 is an endocannabinoid receptor that belongs to group δ of the rhodopsin-like (class A) receptors. Like all other G-protein coupled receptors, it consists of seven trans-membrane helixes, intracellular C-terminal domain and extracellular N-terminal extension1.The other known cannabinoid receptors, CB1 and CB2 share low homology with GPR55 and have different ligand specificity and different downstream activated cascade. Thus, GPR55 consists on its own a class of cannabinoid receptor2.GPR55 is highly expressed in the striatum and binds a wide range of ligands, among which are endocannabinoids: Anadanine and 2-Arachidonoyl Glycerol, and phytocannabinoids: Tetrahydrocannabinol (THC), abnormal cannabinoid (CBD) and cannabidiol3. Following ligand binding, GPR55 couples to Gα12,13 proteins, which eventually lead to increased intracellular Ca2+ levels2.The endocannabinoid system has a tremendous potential for experimental and clinical research, based on their evolutionary conservation and role in fundamental physiological and pathological processes, including pain modulation, immune function, neuroprotection, cancer, cardiovascular diseases, fertility and appetite4. Clinical studies using cannabinoid receptor agonists and antagonists have suggested their possible use in modulating acute and/or chronic diseases such as hypertension, glaucoma, emesis, anxiety, depression, gastrointestinal and hepatic disorders, ulcerative colitis and neuropathic and inflammatory pain5.

Alternative names: G-protein coupled receptor 55

Gen-ID: 9290

NCBI Accession: NM_005683

UniProt: Q9Y2T6