GPR4 Antikörper (Extracellular)

Produktnummer ABIN7043160

Dieses Anti-GPR4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von GPR4 in WB, IHC, FACS und LCI. Geeignet für Ratte.

Kurzübersicht für GPR4 Antikörper (Extracellular) (ABIN7043160)

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR4 Antikörper

Bindungsspezifität: AA 162-177, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR4

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse,human - 15,16 amino acid residues identical

Produktmerkmale: Anti-GPR4 (extracellular) Antibody (ABIN7043160, ABIN7044423 and ABIN7044424) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize GPR4 from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RDRYNHTF(S)FEKFPME, corresponding to amino acid residues 162-177 of rat GPR4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:400

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235415)

Blocking Peptide: (ABIN7235415)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR4

Target: GPR4 (G Protein-Coupled Receptor 4 (GPR4))

Andere Bezeichnung: GPR4

Hintergrund: Acid-sensing G-protein coupled receptor 4, GPR19,G-protein coupled receptor 4 (GPR4) belongs to a protein family comprised of 3 related G-protein coupled receptors (GPCRs): GPR4, OGR1/GPR68 and TDAG8/GPR65. Like all GPCRs, GPR4's structure is composed of seven-transmembrane-spanning domains linked by an N-terminal extracellular domain and a C-terminal intracellular domain2.GPR4 responds to pH changes and is activated by a physiological increase in extracellular H+ through several signaling pathways including- the G12/13-protein/Rho, the Gq/PLC, and the Gs-protein/cAMP pathway1,2. It is fully activated at pH levels of 6.8 and lower and is inactive at pH levels of 7.5 and higher3,4.Recent studies show that the pH -sensing GPCRs also have the ability to regulate cancer cell metastasis and proliferation, immune cell function, inflammation, blood vessel formation, endothelial barrier function thus making them an important therapeutic target for different diseases. Overexpression of GPR4 in human cancer cells shows involvement in driving or maintaining tumor formation.GPR4 is expressed in several tissues including lung, vascular endothelial cells, kidney, heart, liver, skeletal muscle, ovary, and placenta.

Alternative names: GPR4, Acid-sensing G-protein coupled receptor 4, GPR19

Gen-ID: 308408

NCBI Accession: NM_005282

UniProt: Q4KLH9