GPR39 Antikörper (Extracellular)

Produktnummer ABIN7043159

Produktdetails anti-GPR39 Antikörper

Target: GPR39 (G Protein-Coupled Receptor 39 (GPR39))

Bindungsspezifität: AA 309-320, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR39 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR39

Spezifität: 3rd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: rat - 9,Human - 11,12 amino acid residues identical

Produktmerkmale: Anti-GPR39 (extracellular) Antibody (ABIN7043159, ABIN7044429 and ABIN7044430)) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and can potentially be used for detecting the protein in living cells. It has been designed to recognize GPR39 from human, mouse, and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KPKHDWTRTYFR, corresponding to amino acid residues 309-320 of mouse GPR39

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-GR045

Blocking Peptide: BLP-GR045

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR39

Target: GPR39 (G Protein-Coupled Receptor 39 (GPR39))

Andere Bezeichnung: GPR39 (GPR39 Produkte)

Hintergrund: G-protein coupled receptor 39, Zinc receptor GPR39,GPR39 (G-protein coupled receptor 39) is member of the rhodopsin-like family of 7-transmembrane containing GPCRs and is closely related to the ghrelin/neurotensin receptor subfamily.The human GPR39 gene encodes the full-length receptor (GPR39-1a). GPR39 is expressed in various tissues including adipose tissue, heart and thyroid but is most abundant in the pancreas, GI tract, liver and kidney. The GPR39 receptor is comprised of an extracellular N-terminus, seven membrane spanning loops, and an intracellular C-terminus. It also contains four cysteine residues in its extracellular domains. These have been shown to form two disulfide bridges which are conserved within all vertebrate GPR39 sequences with the exception that the first bridge is absent from the fish receptors.Although GPR39 has a high degree of constitutive activity mediated through Gαq and Gα12/13 pathways, it has been discovered recently that Zn2+ serves as a ligand capable of activating the receptor. Following Zinc activation, both pathways are significantly enhanced and signaling occurs through the Gαs pathway as well.GPR39 has been implicated in several pathologies: Disruption of the GPR39 gene is associated with impaired insulin secretion. GPR39 knockout mice exhibit decreased insulin levels and lower levels of insulin promoting factor-1 and HNF-1α in pancreatic islets. GPR39 knockout mice also exhibit lower levels of CREB and BDNF proteins that are important in neuronal plasticity and antidepressant response thus leading to depressive-like behaviors in animals.

Alternative names: GPR39, G-protein coupled receptor 39, Zinc receptor GPR39

Gen-ID: 71111

NCBI Accession: NM_001508

UniProt: Q5U431

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis, Feeding Behaviour