GPR34 Antikörper (2nd Extracellular Loop)

Produktnummer ABIN7886445

Der Kaninchen Polyklonal anti-GPR34 Antikörper wird verwendet zum Nachweis von GPR34 in Proben von Maus. Er wurde validiert für WB und FACS.

Kurzübersicht für GPR34 Antikörper (2nd Extracellular Loop) (ABIN7886445)

Target: GPR34 (G Protein-Coupled Receptor 34 (GPR34))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR34 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-GPR34 Antikörper

Bindungsspezifität: 2nd Extracellular Loop, AA 197-209

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR34

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,rat - 12,13 amino acid residues identical

Produktmerkmale: Anti-GPR34 (extracellular) Antibody (ABIN7043153, ABIN7044449 and ABIN7044450) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and indirect flow cytometry applications. It has been designed to recognize GPR34 from human, mouse, and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: CFHYRDRHNAKGE, corresponding to amino acid residues 197 - 209 of mouse GPR34

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235396)

Blocking Peptide: (ABIN7235396)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR34

Target: GPR34 (G Protein-Coupled Receptor 34 (GPR34))

Andere Bezeichnung: GPR34

Hintergrund:

Synonyms: G-protein coupled receptor 34, LYPSR1

Description: The G-protein-coupled receptor 34 (GPR34) is currently classified as an orphan receptor of the P2Y12-like receptor group. The receptor belongs to the super family of G-protein coupled receptors (GPCRs)1.The N-terminus of human GPR34 has three putative N-glycosylation sites (NXS/T), and the C-terminus has a cysteine residue which is palmitoylated in many GPCRs. GPR34 contains several putative phosphorylation sites primed by a serine or threonine in the C-terminus2.GPR34 is highly expressed in immune cells such as mast cells, macrophages, microglia, and immune system-derived cell lines (HL-60, K562, WEHI-3B, RAW 264.7, P815). Studies shows that GPR34 function is required to arrest microglia in the M0 homeostatic non-phagocytic phenotype and downregulation of GPR34 promotes the neurodegenerative microglia phenotype2,3,4.GPR34 is linked to several human diseases including immunological diseases and cancer. Most known effects of GPR34 are immune function-related. For example, GPR34 is significantly down-regulated in Mycobacterium tuberculosis-infected macrophages, GPR34 mRNA is significantly lower in the cerebrospinal fluid from multiple sclerosis patients, GPR34 is significantly upregulated in the striatum and cortex of patients with Parkinson's disease but downregulated in substantia nigra. elevated GPR34 also plays a role in the progression of some tumors, such as human gastric adenocarcinoma, mucosa-associated lymphoid tissue (MALT) lymphoma, and colorectal cancer1-3.

Gen-ID: 23890

NCBI Accession: NM_005300

UniProt: Q9R1K6