GPR171 Antikörper (2nd Extracellular Loop)

Produktnummer ABIN7886444

Der Kaninchen Polyklonal anti-GPR171 Antikörper wird verwendet zum Nachweis von GPR171 in Proben von Maus. Er wurde validiert für WB, FACS und LCI.

Kurzübersicht für GPR171 Antikörper (2nd Extracellular Loop) (ABIN7886444)

Target: GPR171 (G Protein-Coupled Receptor 171 (GPR171))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR171 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-GPR171 Antikörper

Bindungsspezifität: 2nd Extracellular Loop, AA 155-169

Verwendungszweck: A Rabbit Polyclonal Antibody to GPR171

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,15 amino acid residues identical, rat - 13,human - 14

Produktmerkmale: Anti-GPR171 (extracellular) Antibody (ABIN7043150, ABIN7044447 and ABIN7044448) is a highly specific antibody directed against an epitope of the mouse protein. The antibody can be used in western blot and live cell flow cytometry. It has been designed to recognize GPR171 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: Peptide (C)KDIKEKSNVG(S)MEFK, corresponding to amino acid residues 155 - 169 of mouse GPR171.

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235380)

Blocking Peptide: (ABIN7235380)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu GPR171

Target: GPR171 (G Protein-Coupled Receptor 171 (GPR171))

Andere Bezeichnung: GPR171

Hintergrund:

Synonyms: G-Protein Coupled Receptor 171, Platelet Activating Receptor Homolog, G-protein coupled receptor H963

Description: G protein-coupled receptor 171 (GPR171) is an orphan G-protein coupled receptor (GPCR) activated by BigLEN, one of the most abundant neuropeptides in the brain that is derived from ProSAAS1. GPR171 contains seven transmembrane domains, an extracellular N-terminus and an intracellular C-terminal tail. Its detailed structure and ligand binding domain structure was predicted by homology modeling based on its similarity to P2Y12 receptor2,3.BigLEN-GPR171 neuropeptide-receptor system is expressed in the brain, at the basolateral amygdala (BLA) and the periaqueductal gray brain area4-6.This system plays an important role in regulating feeding-related behaviors, food intake and body weight regulation. It is also involved in anxiety behavior and various psychiatric disorders4,5.Furthermore, BigLEN-GPR171 are expressed in GABAergic neurons within the periaqueductal gray, a key brain area involved in pain modulation and opioid functions6.GPR171 is a potential target for drug development in obesity and weight problems, anxiety, various psychiatric disorders and pain.

Gen-ID: 229323

NCBI Accession: NM_013308

UniProt: Q8BG55