FZD3 Antikörper (Intracellular)

Produktnummer ABIN7043142

Dieses Anti-FZD3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von FZD3 in WB und IHC. Geeignet für Maus.

Kurzübersicht für FZD3 Antikörper (Intracellular) (ABIN7043142)

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FZD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-FZD3 Antikörper

Bindungsspezifität: AA 581-596, Intracellular

Verwendungszweck: A Rabbit Polyclonal antibody to Frizzled-3 receptor.

Spezifität: Intracellular, C-terminus.

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,rat - identical

Produktmerkmale: A rabbit polyclonal antibody to Frizzled-3 receptor.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: Peptide (C)KVSSYHGSLHRSRDGR, corresponding to amino acid residues 581 - 596 of mouse Frizzled-3

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235344)

Blocking Peptide: (ABIN7235344)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu FZD3

Target: FZD3 (Frizzled Family Receptor 3 (FZD3))

Andere Bezeichnung: FZD3

Hintergrund: Frizzled Class Receptor 3, Fz-3,Frizzled 3, encoded by FZD3 gene, is a receptor in the Wnt/β-catenin pathway, a conserved signaling pathway that regulates gene expression and controls diverse developmental processes 1. It belongs to the Frizzled protein family that contains multiple hydrophobic domains predicted to form seven transmembrane helices, similar to G protein coupled receptors. Conserved sequences are found in the Frizzled proteins extracellular cysteine-rich domain (CRD), the putative transmembrane domains, parts of the intracellular loops and a juxtamembrane motif, KTXXXW, found in the membrane-proximal C-terminal region of all Frizzleds. The C-terminal tails, however, are highly divergent 2. Frizzled-3 is constitutively expressed in murine melanocyte precursors, melanocytes and human melanocytes 3, it is also expressed in the epidermis of the developing embryo 4 and its expression levels are changing between different parts of the ovary throughout the process of embryogenesis 1. Because of its crucial role in regulating proper embryogenesis and its role in the development of the central nervous system, including synaptogenesis and structural plasticity, mutations in FZD3 gene were shown to have a significant association with the development of schizophrenia and other psychotic disorders 5.

Alternative names: Frizzled Class Receptor 3, Fz-3

Gen-ID: 14365

NCBI Accession: NM_145866

UniProt: Q61086

Pathways: WNT Signalweg, Tube Formation