FFAR2 Antikörper (Intracellular)

Produktnummer ABIN7043138

Produktdetails anti-FFAR2 Antikörper

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Bindungsspezifität: AA 301-314, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser FFAR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Free Fatty Acid Receptor 2 (GPR43)

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Will not recognize human samples.

Homologie: Mouse -12,14 amino acid residues identical

Produktmerkmale: Anti-GPR43/FFAR2 Antibody (ABIN7043138, ABIN7044289 and ABIN7044290)) is a highly selective antibody directed against an epitope of the rat Free fatty acid receptor 2. The antibody can be used in western blot analysis. It has been designed to recognize FFAR2 from mouse and rat samples. The antibody is not suited to recognize FFAR2 from human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RGAEETVEGTKTDR, corresponding to amino acid residues 301-314 of rat FFAR2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:200-1:500

Kommentare:

Cited Application: IFC

Negative Control: BLP-FR032

Blocking Peptide: BLP-FR032

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu FFAR2

Target: FFAR2 (Free Fatty Acid Receptor 2 (FFAR2))

Andere Bezeichnung: FFAR2 (FFAR2 Produkte)

Hintergrund: G-protein coupled receptor 43, Free fatty acid receptor 2,Fatty acids have long been recognized for the variety of their effects in the body. Yet, until recently, these actions were thought to be mediated exclusively via actions on cellular metabolism. There are currently three known receptors for free fatty acids: FFA1, FFA2, and FFA3.Free fatty acid receptor 2 (FFA2) is a G-protein coupled receptor containing seven hydrophobic regions, consistent with transmembrane (TM) spanning helices. FFA2 contains cysteine residues in the first and second extracellular loops that are likely to contribute to structure via the formation of intramolecular disulfide bonds. FFA2 mRNA is detected in a number of tissues, but the highest expression is found in immune cells such as neutrophils, monocytes, peripheral blood mononuclear cells, B-lymphocytes, and polymorphonuclear cells.Short chain fatty acids (SCFAs) are fatty acids of less than six carbons and were found to be endogenous agonists of FFA2. The most potent agonist of the receptor is acetate (C2) and propionate (C3) being equipotent followed by butyrate (C4) and then valerate (C5) and formate (C1)1.FFA2 is implicated in the pathogenesis of several diseases. Stimulation of FFA2 by acetate allows resolution of a colitis-related inflammatory response. FFA2 knockout mice show exacerbated or unresolved inflammation in cases of acute and chronic colitis, arthritis, and asthma. In contrast, other studies show that knockout mice exhibit an increased mortality compared to control mice, despite reduced immune cell recruitment, decreased colonic inflammation, and attenuated colonic tissue damage2.

Alternative names: GPR43/FFAR2, G-protein coupled receptor 43, Free fatty acid receptor 2

Gen-ID: 292794

NCBI Accession: NM_005306

UniProt: Q76EI6