PIEZO2 Antikörper (AA 1092-1104)

Produktnummer ABIN7043133

Produktdetails anti-PIEZO2 Antikörper

Target: PIEZO2 (FAM38B) (Family with Sequence Similarity 38, Member B (FAM38B))

Bindungsspezifität: AA 1092-1104

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser PIEZO2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Piezo2

Spezifität: Located in an internal region of human Piezo2 (the exact topology of Piezo2 remains to be defined).

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat - 12,13 amino acid residues identical

Produktmerkmale: Anti-Piezo2 Antibody (ABIN7043133, ABIN7044990 and ABIN7044991) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot analysis. It has been designed to recognize Piezo2 from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Güteklasse: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RTIFHDITRLHLD, corresponding to amino acid residues 1092 - 1104 of human Piezo2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:200

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IP|IHC|ICC|Whole Tissue Staining

Negative Control: (ABIN7235322)

Blocking Peptide: (ABIN7235322)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu PIEZO2

Target: PIEZO2 (FAM38B) (Family with Sequence Similarity 38, Member B (FAM38B))

Andere Bezeichnung: PIEZO2 (FAM38B Produkte)

Hintergrund: Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B,Piezo-type mechanosensitive ion channel component 2 (Piezo2) is a homotrimeric ion channel. Piezo2 depicts rapidly adapting mechanically activated (MA) currents in somatosensory neurons.Piezo1 and Piezo2 comprise the Piezo protein family. These are large transmembrane proteins are conserved among various species, and all have between 24 and 36 predicted transmembrane domains. Both channels open in response to mechanical stimuli, allowing positively charged ions, including calcium, to flow into the cell1.Piezo2 protein contains over 2,800 residues, it forms a three-bladed, propeller like trimer that comprises 114 transmembrane helices (38 per protomer). Transmembrane helices 1-36 (TM1-36) are folded into nine tandem units of four transmembrane helices each to form the unusual non-planar blades2.Piezo2 has an essential role in sensing gentle touch, tactile pain, airway stretch and lung inflation. It also serves as a baroreceptor for sensing blood pressure and regulating heart rate. Mutations in human Piezo2 are associated with distal arthrogryposis, a disorder characterized by joint deformities (contractures) that restrict movement in the hands and feet3,4.

Alternative names: Piezo-Type Mechanosensitive Ion Channel Component 2, Protein FAM38B

Gen-ID: 63895

NCBI Accession: NM_022068

UniProt: Q9H5I5