NBC4 Antikörper (Intracellular)

Produktnummer ABIN7043118

Produktdetails anti-NBC4 Antikörper

Target: NBC4 (Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4))

Bindungsspezifität: AA 11-23, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser NBC4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: A Rabbit Polyclonal Antibody to NBC4 Transporter

Spezifität: Intracellular, N-terminus

Kreuzreaktivität (Details): Not recommended for human and mouse samples.

Homologie: Rat only

Produktmerkmale: Anti-Rat SLC4A5 (NBC4) Antibody is directed against an epitope of rat NBCe2. Anti-Rat SLC4A5 (NBC4) Antibody (ABIN7043118, ABIN7044819 and ABIN7044820)) can be used in western blot analysis. It has been designed to recognize NBCe2 from rat samples. The antibody is not recommended for mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KLEPTSYRRRHPE, corresponding to amino acid residues 11-23 of rat NBCe2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Kommentare:

Cited Application: IFC

Negative Control: BLP-NT076

Blocking Peptide: BLP-NT076

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu NBC4

Target: NBC4 (Electrogenic Sodium Bicarbonate Cotransporter 4 (NBC4))

Andere Bezeichnung: SLC4A5 (NBC4 Produkte)

Hintergrund: Electrogenic sodium bicarbonate cotransporter 4, NBCe2,The SLC4 transporter family, also known as the bicarbonate (HCO3-)-transporter family, includes the products of ten human genes (SLC4A1-5, A7-11). In mammals, the SLC4 membrane proteins are critical for several physiological processes, including the carriage of carbon dioxide (CO2) from the systemic capillaries to the pulmonary capillaries, the regulation of cell volume in multiple cell types, and the regulation of intracellular pH in nearly every cell of the body1.NBCe2 (NBC4) is an electrogenic sodium bicarbonate co-transporter encoded by SLC4A5. There are six NBCe2 isoform (SLC4A5A-F) reported2. NBCe2 is an integral membrane protein consisting of 14 transmembrane domains. NBCe2 has a long N-terminal hydrophilic domain and a much shorter C-terminal hydrophilic domain, both of which are intracellular3.NBCe2 is predominantly detected in the brain (highest in prefrontal cortex), epididymis, cardiac muscle, smooth muscle, kidney, and choroid plexus/apical1.Several studies identified SLC4A5 as a positional candidate for hypertension4,5. In mice, disruption of slc4a5 causes both arterial hypertension as well as metabolic acidosis (distal renal tubular acidosis)6. In addition, mice lacking NBCe2 have reduced choroid plexus secretion and reduced ventricle size. These combined issues change intracranial pressure, CSF electrolytes, and cause severe eye pathologies such as photoreceptor loss, ganglion cell loss, and retinal detachment7.

Alternative names: SLC4A5 (NBC4), Electrogenic sodium bicarbonate cotransporter 4, NBCe2

Gen-ID: 297386

NCBI Accession: NM_001386136

UniProt: Q6RI88