EFCAB4B Antikörper (Intracellular)

Produktnummer ABIN7043114

Produktdetails anti-EFCAB4B Antikörper

Target: EFCAB4B (EF-Hand Calcium Binding Domain 4B (EFCAB4B))

Bindungsspezifität: AA 245-258, Intracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser EFCAB4B Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Verwendungszweck: A Rabbit Polyclonal Antibody to CRAC Channel Regulator 2A

Spezifität: Intracellular

Kreuzreaktivität: Human, Ratte

Homologie: Rat - 13,14 amino acid residues identical

Produktmerkmale: Anti-CRACR2A (EFCAB4B) Antibody is directed against an epitope of the human CRAC channel regulator 2A. Anti-CRACR2A (EFCAB4B) Antibody (ABIN7043114, ABIN7044115 and ABIN7044116)) can be used in western blot analysis. It has been designed to recognize CRAC channel regulator 2A from rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)KSEKEQFLLKDTER, corresponding to amino acid residues 245-258 of human CRACR2A

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: BLP-CC324

Blocking Peptide: BLP-CC324

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu EFCAB4B

Target: EFCAB4B (EF-Hand Calcium Binding Domain 4B (EFCAB4B))

Andere Bezeichnung: CRACR2A (EFCAB4B Produkte)

Hintergrund: CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B,Calcium is a vital ion for the functioning of many intracellular processes. When Ca2+ stored in the endoplasmic reticulum is depleted, there is an activation of store-operated channels that results in the influx of Ca2+ from outside the cell. In non-excitable cells and especially in immune cells, most of this influx is carried out by calcium release activated calcium channels also known as "CRACs"1.Mediation of signals from the ER to CRACs and activation of the CRAC channel is conducted by two modulators that physically cluster with the CRAC channel known as Orai1 and STIM1. STIM1 is a single transmembrane segment protein that has a long C-terminal cytoplasmic region and an N-terminus which detects ER Ca2+ depletion. Orai1 is a macromolecular complex with linkers of varying lengths between the ER and plasma membranes.Novel findings suggest the existence of a complex comprised of Orai1, STIM1 and additional proteins. One of these proteins is an EF-hand protein named CRACR2A which regulates Orai-STIM interaction. An EF-hand is a structural domain found in Ca2+ binding proteins consisting of two alpha helices linked by a short loop region.CRACR2A interacts with the cytoplasmic regions of both Orai1 and STIM1 thus creating a tertiary complex. Under high concentrations of Ca2+, CRACR2A caused a dissociation of Orai1 and STIM1 and a mutated CRACR2A was found, in one study, to cause elevated Ca2+ levels that caused cell death in T cells2.Further research is required to fully determine the activation and regulation mechanisms of CRACs and this can be performed by better understanding CRACR2A and its binding proteins.

Alternative names: CRACR2A (EFCAB4B), CRAC channel regulator 2A, Calcium release-activated calcium channel regulator 2A, EF-hand calcium-binding domain-containing protein 4B, EFC4B

Gen-ID: 84766

NCBI Accession: NM_032680

UniProt: Q9BSW2