DRD3 Antikörper (Extracellular)

Produktnummer ABIN7043109

Produktdetails anti-DRD3 Antikörper

Target: DRD3 (Dopamine Receptor D3 (DRD3))

Bindungsspezifität: AA 15-29, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DRD3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to D3 Dopamine Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 12,15 amino acid residues identical

Produktmerkmale: Anti-D3 Dopamine Receptor (extracellular) Antibody is directed against an epitope located at the extracellular N-terminus of the rat D3 dopamine receptor. Anti-D3 Dopamine Receptor (extracellular) Antibody (ABIN7043109, ABIN7044231 and ABIN7044232)) can be used in western blot analysis and immunohistochemical applications, and recognizes DRD3 from rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Güteklasse: KO Validated

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CGAENSTGVNRARPH, corresponding to amino acid residues 15-29 of rat DRD3 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC|IFC

Negative Control: BLP-DR003

Blocking Peptide: BLP-DR003

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu DRD3

Target: DRD3 (Dopamine Receptor D3 (DRD3))

Andere Bezeichnung: DRD3 (DRD3 Produkte)

Hintergrund: DRD3,The D3 Dopamine Receptor (D3 receptor) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and endocrine regulation. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and schizophrenia.1All five dopamine receptors belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes).1The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity.1The D3 receptor distribution in the brain is relatively restricted to limbic areas such as striatum, islands of Calleja and olfactory tubercle. In the periphery, it is expressed in the kidney particularly in proximal tubules.1The exact physiological function of the D3 receptor remains to be fully elucidated. In studies using D3 receptor knockout mice, the most prominent dysfunction was the development of rennin-dependent hypertension. Potential roles in reinforcement and reward behaviors have also been suggested as well as roles in neuropsychiatric disorders such as drug abuse and schizophrenia.2

Alternative names: D3 Dopamine Receptor, DRD3

Gen-ID: 29238

NCBI Accession: NM_000796

UniProt: P19020

Pathways: Regulation of Systemic Arterial Blood Pressure by Hormones, cAMP Metabolic Process, Regulation of G-Protein Coupled Receptor Protein Signaling, Proton Transport, Negative Regulation of Transporter Activity