Dopamine Receptor d1 Antikörper (Intracellular)

Produktnummer ABIN7043106

Produktdetails anti-Dopamine Receptor d1 Antikörper

Target: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Bindungsspezifität: AA 372-385, Intracellular

Reaktivität: Ratte

Wirt: Meerschweinchen

Klonalität: Polyklonal

Konjugat: Dieser Dopamine Receptor d1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck: A Guinea Pig Polyclonal Antibody to D1 Dopamine Receptor

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,dog,pig - identical, human - 13,14 amino acid residues identical

Produktmerkmale: Guinea pig Anti-D1 Dopamine Receptor Antibody is directed against an epitope of rat D1 Receptor. Guinea pig Anti-D1 Dopamine Receptor Antibody (#) raised in guinea pigs can be used in western blot and immunohistochemistry applications. It has been designed to recognize DRD1 from rat, human and mouse samples. The antigen used to immunize guinea pigs is the same as Anti-D1 Dopamine Receptor Antibody (ABIN7043105, ABIN7044227 and ABIN7044228)) raised in rabbit. Our line of guinea pig antibodies enables more flexibility with our products such as multiplex staining studies, immunoprecipitation, etc.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: SSHHEPRGSISKDC, corresponding to amino acid residues 372-385 of rat DRD1 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: BLP-DR001

Blocking Peptide: BLP-DR001

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu Dopamine Receptor d1

Target: Dopamine Receptor d1 (DRD1) (Dopamine Receptor D1 (DRD1))

Andere Bezeichnung: DRD1 (DRD1 Produkte)

Hintergrund: DRD1, D(1A) dopamine receptor,The D1 dopamine receptor (D1 receptor, DRD1) is one of five receptors that mediate the effects of the catecholamine neurotransmitter dopamine. Dopamine regulates a variety of functions including locomotor activity, emotion, positive reinforcement, food intake, and hormone secretion. The dopaminergic system has been extensively studied in the last thirty years mainly because its dysregulation has been linked to several neurological and neuropsychiatric diseases including Parkinson's disease and Schizophrenia1.All five dopamine receptors belong to the 7-transmembrane domain, G-protein coupled receptor (GPCR) superfamily.Historically, the five receptors have been divided into two subfamilies based on pharmacological and structural considerations: the D1-like subfamily (that includes the D1 and D5 subtypes) and the D2-like subfamily (that includes the D2-, D3- and D4 subtypes)1.The D1-like receptors are coupled to Gs-type G proteins and enhance adenylate cyclase activity while the D2-like receptors are coupled to Gi-type G proteins and inhibit adenylate cyclase activity1.The D1 receptor is widely distributed throughout the brain with the highest expression in the cerebral cortex and striatum. In the periphery the D1 receptor has been detected in the adrenal cortex, kidney and heart.Functionally, the D1 receptor has been implicated in the regulation of both locomotor and cognitive functions including the maintaining of spontaneous motor behaviors, the control of working memory and cognition as well as the regulation of craving and reward pathways.In addition, the D1 receptor plays an important role in the pathogenesis of hypertension by regulating epithelial Na+ transport and by interacting with vasoactive hormones/humoral factors, such as aldosterone and angiotensin1,2.

Alternative names: D1 Dopamine Receptor, DRD1, D(1A) dopamine receptor

Gen-ID: 24316

NCBI Accession: NM_000794

UniProt: P18901

Pathways: cAMP Metabolic Process, Inositol Metabolic Process, Protein targeting to Nucleus, Feeding Behaviour, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity