DPP10 Antikörper (Extracellular)

Produktnummer ABIN7043099

Produktdetails anti-DPP10 Antikörper

Target: DPP10 (Dipeptidylpeptidase 10 (DPP10))

Bindungsspezifität: AA 356-370, Extracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser DPP10 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Verwendungszweck: A Rabbit Polyclonal Antibody to Dipeptidyl Peptidase 10

Spezifität: Extracellular, C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: 15 amino acid residues identical,Mouse - 14, human - 12

Produktmerkmale: Anti-DPP10 (extracellular) Antibody (ABIN7043099, ABIN7045044 and ABIN7045045)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. The antibody recognizes an extracellular epitope and is thus ideal for detecting the protein in living cells. It has been designed to recognize DPP10 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: CSRKYEMTSDTWISK, corresponding to amino acid residues 356-370 of rat DPP10

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:1000

Application Dilutions Western blot wb: 1:200-1:400

Kommentare:

Cited Application: IP

Negative Control: BLP-PC147

Blocking Peptide: BLP-PC147

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu DPP10

Target: DPP10 (Dipeptidylpeptidase 10 (DPP10))

Andere Bezeichnung: DPP10 (DPP10 Produkte)

Hintergrund: Inactive dipeptidyl peptidase 10, Dipeptidyl peptidase X, DPP X, DPPY, DPL2, DPRP3,Dipeptidyl peptidase 10 (DPP10, DPPY) is a member of the prolyl oligopeptidase family (shares a 30 % sequence identity with DPP4) and belongs to the S9B serine protease subfamily. DPP10 is a homologous glycosylated, single-pass type II transmembrane protein that lacks the crucial serine residue of the catalytic triad. Unlike other KV4 subunits such as DPP6, DPP10 has a restricted expression pattern including the brain, adrenal gland and pancreas. The presence of DPP10 in endocrine cells suggests that the protein may also have an additional function related to the regulation of hormone secretion. DPP10 accelerates the activation and inactivation of KV4.3 channel gating with distinct biophysical properties.DPP10 associates with the α-subunits of the KV4 subfamily to form a ternary complex of approximately 750 kDa1. In addition, DPP10 forms complexes with other auxiliary subunits of the KV4 channels. In dorsal root ganglion DPP10 is co-localized with K+ channel-interacting proteins 1 and 2 (KChIPs) in the somatic surface and cytoplasm of KV4.3 nociceptors. Recently it has been hypothesized that KV4/KChIP/DPPL ternary complexes exist in subthreshold A-type K+ currents (Isas) expressing nociceptors and pain-modulating spinal interneurons2.DPP10 has been implicated in various pathologies. Aggregation of the protein is associated with neurodegenerative conditions such as Alzheimer's, diffuse Lewy body disease and fronto-temporal dementia3.

Alternative names: DPP10, Inactive dipeptidyl peptidase 10, Dipeptidyl peptidase X, DPP X, DPPY, DPL2, DPRP3

Gen-ID: 363972

NCBI Accession: NM_020868

UniProt: Q6Q629