CasLTR2 Antikörper (3rd Extracellular Loop)

Produktnummer ABIN7886534

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CasLTR2 in WB und FACS. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CasLTR2 Antikörper (3rd Extracellular Loop) (ABIN7886534)

Target: CasLTR2 (CYSLTR2) (Cysteinyl Leukotriene Receptor 2 (CYSLTR2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CasLTR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-CasLTR2 Antikörper

Bindungsspezifität: 3rd Extracellular Loop, AA 270-284

Verwendungszweck: A Rabbit Polyclonal Antibody to Human CysLTR2

Kreuzreaktivität (Details): Not recommended for use with rat and mouse samples.

Homologie: Human only

Produktmerkmale: Anti-Human CysLTR2 (extracellular) Antibody (ABIN7043095 and ABIN7044560) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CysLTR2 from human samples only.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HLTTWKVGL(S)KDRLH, corresponding to amino acid residues 270-284 of human CysLTR2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CasLTR2

Target: CasLTR2 (CYSLTR2) (Cysteinyl Leukotriene Receptor 2 (CYSLTR2))

Andere Bezeichnung: CYSLTR2

Hintergrund:

Synonyms: CYSLT2, Cysteinyl leukotriene receptor 2, hGPCR21, G-protein coupled receptor HG57

Description: LTC4, LTD4, and LTE4 are cysteinyl leukotrienes (cys-LTs) peptide conjugated lipids derived from arachidonic acid as a result of immune or inflammatory stimuli1-3.The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE45.Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14.Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6.

Gen-ID: 57105

NCBI Accession: NM_001308465

UniProt: Q9NS75