CysLTR1 Antikörper (3rd Extracellular Loop)

Produktnummer ABIN7886533

Der Kaninchen Polyklonal anti-CysLTR1 Antikörper wird verwendet zum Nachweis von CysLTR1 in Proben von Human. Er wurde validiert für WB und FACS.

Kurzübersicht für CysLTR1 Antikörper (3rd Extracellular Loop) (ABIN7886533)

Target: CysLTR1 (CYSLTR1) (Cysteinyl Leukotriene Receptor 1 (CYSLTR1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CysLTR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS)

Produktdetails anti-CysLTR1 Antikörper

Bindungsspezifität: 3rd Extracellular Loop, AA 261-275

Verwendungszweck: A Rabbit Polyclonal Antibody to CysLTR1

Kreuzreaktivität: Human, Maus

Homologie: mouse - 12,Human - 14,15, rat - 11,15 amino acid residues identical

Produktmerkmale: Anti-CysLTR1 (extracellular) Antibody (ABIN7043093, ABIN7044558 and ABIN7044559) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CysLTR1 from human, mouse, and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HNETKP(S)DSVLRMQK, corresponding to amino acid residues 261-275 of human CysLTR1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 1:50

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235196)

Blocking Peptide: (ABIN7235196)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CysLTR1

Target: CysLTR1 (CYSLTR1) (Cysteinyl Leukotriene Receptor 1 (CYSLTR1))

Andere Bezeichnung: CYSLTR1

Hintergrund:

Synonyms: CYSLT1, Cysteinyl leukotriene receptor 1, Cysteinyl leukotriene D4 receptor, LTD4 receptor, G-protein coupled receptor HG55, HMTMF81

Description: LTC4, LTD4, and LTE4 are leukotrienes (cys-LTs) derived from arachidonic acid as a result of immune or inflammatory stimuli1-3.The above mentioned cys-LTs bind and activate two receptors belonging to the G-protein coupled receptor superfamily, CysLTR1 and CysLTR2 (also named cysLT1 and cysLT2 respectively). There is evidence though that other receptors are yet to be identified4. These two receptors couple to both pertussis toxin sensitive (Gi/o) and insensitive (Gq, which initiates Ca2+ influx) G-proteins for their signaling. The G-protein coupled to is probably determined by which ligand binds the receptor as all three cys-LTs can activate the two receptors albeit with different affinities5. In mouse, CysLTR1 and CysLTR2 are subject to alternative splicing. CysLTR1 receptor expresses greater affinity for LTD4 and binds equally to LTC4 and LTE4 whereas CysLTR2 has greater and equal affinity towards LTC4 and LTD4 over LTE45.Human CysLTR1 is highly expressed in spleen, peripheral blood, leukocytes. It is less strongly expressed in lung, small intestine, pancreas and hardly or not expressed in liver, colon, kidney, skeletal muscle, testis heart and brain4. On the other hand, CysLTR2 is highly expressed in spleen and leukocytes. Its expression in the heart, brain, spinal cord and adrenal gland differs it from that of CysLTR14.Cys-LTs and their receptors are involved in respiratory diseases like asthma and other conditions such as cancer cardiovascular, gastrointestinal, skin and immune disorders1,6.

Gen-ID: 10800

NCBI Accession: NM_001282186

UniProt: Q9Y271