CNGA3 Antikörper (Intracellular)

Produktnummer ABIN7043091

Dieses Anti-CNGA3-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CNGA3 in WB, IHC und IF. Geeignet für Ratte.

Kurzübersicht für CNGA3 Antikörper (Intracellular) (ABIN7043091)

Target: CNGA3 (Cyclic Nucleotide Gated Channel alpha 3 (CNGA3))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CNGA3 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Produktdetails anti-CNGA3 Antikörper

Bindungsspezifität: AA 594-611, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to CNGA3 Channel

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Recognizes the two published isoforms of CNGA3.

Homologie: Mouse - 15,18 amino acid residues identical

Produktmerkmale: Anti-CNGA3 Antibody (ABIN7043091 and ABIN7044975) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot and immunohistochemistry applications. It has been designed to recognize CNGA3 from rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)GHGFSPDRENSEDASKAD, corresponding to amino acid residues 594-611 of rat CNGA3 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: ICC

Negative Control: (ABIN7235189)

Blocking Peptide: (ABIN7235189)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CNGA3

Target: CNGA3 (Cyclic Nucleotide Gated Channel alpha 3 (CNGA3))

Andere Bezeichnung: CNGA3

Hintergrund: Cyclic nucleotide-gated cation channel alpha 3, CNG channel alpha 3, CNG3, CNCG3, CNGα2, Cone photoreceptor cGMP-gated channel subunit alpha, CCNC1, ACHM2,Cyclic nucleotides are important second messengers in many cellular functions such as visual transduction, and relaxation of smooth muscle cells. Cyclic nucleotides exert their cellular functions through three major classes of cellular receptors, one of them is the cyclic nucleotide-gated (CNG) channels.1 The CNG channels are non-selective cation channels facilitating the influx of Na+ and Ca2+ ions, following activation by intracellular cyclic nucleotides.2 In vertebrates, six members of the CNG channel family were identified and grouped according to sequence homology into two subtypes, CNGA and CNGB. The native CNG channels are composed of a and b subunits in a tetrameric configuration. To date, four types of the a subunits (CNGA1-4) and two b subunits (CNGB1, CNGB3) have been characterized.3-4 Each subunit contains 6 TM domains and intracellular cAMP or cGMP binding domains but, are also modulated by other factors including phosphorylation and calmodulin.5 In a heterologous expression system, only the a subunits are capable of forming functional homomeric channels. CNG ion channels are essential in visual and olfactory signal transduction. The CNGA3 channel is primarily expressed in cone photoreceptors where it participates in light transduction (daylight and color vision). CNG channels expression was also found in the hippocampus, cortex, Purkinje cells and other neural derived tissues. CNGA3 was also detected in kidney, heart, brain and sperm, where it was thought to have an important role in the control of sperm motility and fertility. However, CNGA3-deficient mice were shown to be as fertile and produce at normal rate as wild types.6

Alternative names: CNGA3, Cyclic nucleotide-gated cation channel alpha 3, CNG channel alpha 3, CNG3, CNCG3, CNGalpha2, Cone photoreceptor cGMP-gated channel subunit alpha, CCNC1, ACHM2

Gen-ID: 85257

NCBI Accession: NM_001298

UniProt: Q9QWN7

Pathways: Phototransduction