CHRNA1 Antikörper (Extracellular)

Produktnummer ABIN7043066

Der Kaninchen Polyklonal Anti-CHRNA1-Antikörper wurde für WB validiert. Er ist geeignet, CHRNA1 in Proben von Ratte zu detektieren.

Kurzübersicht für CHRNA1 Antikörper (Extracellular) (ABIN7043066)

Target: CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CHRNA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-CHRNA1 Antikörper

Bindungsspezifität: AA 22-34, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to Nicotinic Acetylcholine Receptor α1

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human - identical, mouse - 12,13 amino acid residues identical

Produktmerkmale: Anti-Nicotinic Acetylcholine Receptor α1 (CHRNA1) (extracellular) Antibody (ABIN7043066, ABIN7044629 and ABIN7044630) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot analysis. The antibody recognizes an extracellular epitope and is thus ideal for detecting the receptor in living cells. It has been designed to recognize nAChRα1 from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EHETRLVAKLFKD(C), corresponding to amino acid residues 22-34 of rat nAChRalpha1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7234611)

Blocking Peptide: (ABIN7234611)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CHRNA1

Target: CHRNA1 (Acetylcholine Receptor Subunit alpha (CHRNA1))

Andere Bezeichnung: CHRNA1

Hintergrund: NAChR α1, ACHRA, Cholinergic receptor nicotinic alpha 1, SCCMS,Acetylcholine, released by cholinergic neurons, activates two groups of acetylcholine receptors (AChRs), muscarinic AChRs (mAChRs) which belong to the superfamily of G-protein coupled receptors (GPCRs), and nicotinic AChRs (nAChRs) which belong to the ligand-gated ion channel superfamily. nAChRs also respond to nicotine, hence their name1.To date, 17 different but related subunits of nAChRs have been identified and cloned. They consist of α subunits (α1-10), which is responsible for the binding of ligands. In fact, this subunit includes a Cys-loop in the first extracellular domain that is required for agonist binding2. The other subunits responsible for making up the active receptor are the β (β1-4), γ, δ and ε subunits3. Structurally, all subunits have the following: a conserved large extracellular N-terminal domain, 3 conserved transmembrane domains, a variable cytoplasmic loop and a fourth transmembrane domain with a short extracellular C-terminal domain. An active nAChR is generally a heteropentamer (homopentamers also exist) of these various subunits organized around a central pore1.All α subunits are expressed in neuronal cells except for the α1 subunit which is specifically expressed in skeletal muscle. Indeed, two different nAChR structural entities with the following stoichiometry are observed in this tissue: (α1)2β1δγ in fetal muscle cells and (α1)2β1δε at mature neuromuscular synapses3.The α1 extracellular domain contains the main immunogenic region, a region against which a large fraction of autoantibodies against nAChR is directed in the autoimmune disease myasthenia gravis (MG)4. This autoimmune disease is characterized by the malfunction of neuromuscular transmission as a result of nonfunctional nAChRs, leading to defective signaling at the neuromuscular junction5.Due to its central role in muscle contraction and autonomic nervous system, nAChRs have evolved to be important targets of toxins secreted by plants, insects and animals. One prominent toxin affecting α1, in addition to other subunits, is α-bungarotoxin, a snake toxin which blocks nAChRs6.

Alternative names: Nicotinic Acetylcholine Receptor alpha1 (CHRNA1), nAChR alpha1, ACHRA, Cholinergic receptor nicotinic alpha 1, SCCMS

Gen-ID: 79557

NCBI Accession: NM_000079

UniProt: P25108

Pathways: Skeletal Muscle Fiber Development