CLIC1 Antikörper (Intracellular)

Produktnummer ABIN7043056

Dieses Kaninchen Polyklonal-Antikörper erkennt spezifisch CLIC1 in WB, IF und IC. Er zeigt eine Reaktivität gegenüber Human.

Kurzübersicht für CLIC1 Antikörper (Intracellular) (ABIN7043056)

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CLIC1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-CLIC1 Antikörper

Bindungsspezifität: AA 195-208, Intracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to CLIC1 Channel

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse - identical

Produktmerkmale: Anti-CLIC1 Antibody (ABIN7043056 and ABIN7044139) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and immunocytochemistry applications. It has been designed to recognize CLIC1 from mouse, rat, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RGFTIPEAFRGVHR, corresponding to amino acid residues 195-208 of human CLIC1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7235102)

Blocking Peptide: (ABIN7235102)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CLIC1

Target: CLIC1 (Chloride Intracellular Channel 1 (CLIC1))

Andere Bezeichnung: CLIC1

Hintergrund: Cl- intracellular channel 1, Nuclear chloride ion channel 27, NCC27, Chloride channel ABP,Chloride intracellular channel (CLIC) is a family of proteins encoded by six different genes (CLIC1-6)1-3.The localization of the various CLIC channels remains elusive and controversial since they can be either found in a soluble form in the cytoplasm or as integral membrane proteins (in intracellular compartments such as the nuclear membrane or the endoplasmic reticulum for example, or associated with the plasma membrane) via their single transmembrane domain1.When membrane associated, CLIC channels indeed function as ion channels (enabling the passage of Cl- ions). Non-channel functions are also attributed to CLICs and include cell cycle regulation, cell differentiation, and apoptosis. These different roles depend on whether CLICs are membrane bound or cytosolic1.CLIC1, which was first identified in nuclear membranes, is detected in the epithelia of airways, the gall bladder, pancreas, stomach, small intestine and colon4. CLIC1 was also found to be expressed in the endothelium where it plays a role in cell growth and migration5.

Alternative names: CLIC1, Cl- intracellular channel 1, Nuclear chloride ion channel 27, NCC27, Chloride channel ABP

Gen-ID: 1192

NCBI Accession: NM_001288

UniProt: O00299