CMKLR1 Antikörper (2nd Extracellular Loop)

Produktnummer ABIN7886251

Der Kaninchen Polyklonal anti-CMKLR1 Antikörper (ABIN7886251) detektiert spezifisch CMKLR1 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Ratte.

Kurzübersicht für CMKLR1 Antikörper (2nd Extracellular Loop) (ABIN7886251)

Target: CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CMKLR1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-CMKLR1 Antikörper

Bindungsspezifität: 2nd Extracellular Loop, AA 201-215

Verwendungszweck: A Rabbit Polyclonal Antibody to Chemerin Receptor 1

Kreuzreaktivität (Details): Will unlikely recognize CMKLR1 from human and mouse samples.

Homologie: Rat only

Produktmerkmale: Anti-Rat CMKLR1 (extracellular) Antibody (ABIN7043049, ABIN7044179 and ABIN7044180) is a highly specific antibody directed against an epitope of rat Chemerin receptor 1. It can be used in western blot and immunohistochemistry applications. It has been designed to recognize the protein from rat samples only. The antibody is unlikely to recognize the receptor from human and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)HSTHPRTDPVGYSRH, corresponding to amino acid residues 201-215 of rat Chemerin receptor 1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:300

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CMKLR1

Target: CMKLR1 (Chemokine-Like Receptor 1 (CMKLR1))

Andere Bezeichnung: CMKLR1

Hintergrund:

Synonyms: Chemokine-like receptor 1, Cmklr1, Chemerin1, Gpcr27, G-protein coupled receptor ChemR23, CHEMERINR, G-protein coupled chemoattractant-like receptor, G-protein coupled receptor DEZ

Description: Chemerin Receptor 1 belongs to class A G-protein coupled receptors. The receptor is coupled to Gi/o, leading to an increase in intracellular cAMP and Ca2+ following activation by the binding of Chemerin, its endogenous ligand. Like all GPCRs, it has seven transmembrane domains, an extracellular N-terminus and intracellular C-terminal tail1.Chemerin Receptor 1 has numerous names depending on how the gene encoding the receptor was cloned: Chemokine-like receptor 1, CMKLR1, ChemR23 and others all correspond to the same gene/protein1.Expression of Chemerin receptor 1 at the mRNA level is largely detected in adipose tissue, and skin dermal layer. At the protein level, CMKLR1 is expressed at the cell surface of dendritic cells, monocytes and macrophages, smooth muscle cells, adipose tissue, lung, lymph nodes and testis1.A role for the receptor in the inflammatory response has been attributed, however data indicate contradictory roles, proinflammatory and anti-inflammatory functions of the receptor. Expression of Chemerin receptor 1 in adipose tissue clearly establishes its involvement in energy metabolism as an important regulator of adipogenesis1.

Gen-ID: 60669

NCBI Accession: NM_001142343

UniProt: O35786