CCR9 Antikörper (2nd Extracellular Loop)

Produktnummer ABIN7886250

Der Kaninchen Polyklonal anti-CCR9 Antikörper wird verwendet zum Nachweis von CCR9 in Proben von Human. Er wurde validiert für WB, FACS und LCI.

Kurzübersicht für CCR9 Antikörper (2nd Extracellular Loop) (ABIN7886250)

Target: CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCR9 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-CCR9 Antikörper

Bindungsspezifität: 2nd Extracellular Loop, AA 205-218

Verwendungszweck: A Rabbit Polyclonal Antibody to CCR9 Chemokine Receptor

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse,rat - 10,14 amino acid residues identical

Produktmerkmale: Anti-CCR9 (extracellular) Antibody (ABIN7043036, ABIN7044177 and ABIN7044178) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot and indirect live cell flow cytometry applications. It has been designed to recognize CCR9 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DESTKLKSAVLTLK, corresponding to amino acid residues 205-218 of human CCR9

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: 5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7235059)

Blocking Peptide: (ABIN7235059)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CCR9

Target: CCR9 (Chemokine (C-C Motif) Receptor 9 (CCR9))

Andere Bezeichnung: CCR9

Hintergrund:

Synonyms: C-C chemokine receptor type 9, CC-CKR-9, G-protein coupled receptor 28, GPR28, GPR-9-6, CDw199

Description: CCR9 (C-C chemokine receptor type 9) belongs to the β-chemokine receptor family of the G-protein coupled receptor superfamily. Chemokine receptors play a crucial role in cell migration and are considered to be important therapeutic targets for inflammatory diseases and in tumor chemoresistance and metastasis. In addition, high expression of CCR9 has been reported in a wide range of cancers including melanoma, ovarian cancer, lung cancer, breast cancer and colon cancer. Thus, CCR9 is considered to be a potential tumor biomarker in diagnosis and therapy of cancer1,2. Under physiological conditions, CCR9 is mainly expressed in immature T lymphocytes and on the surface of intestinal cells2.CCR9 receptor interacts with its specific ligand- chemokine ligand 25 (CCL25, thymus-expressed chemokine) in a two-step binding mechanism in which the C-terminal region of CCL25 first binds the N-terminus region and extracellular loops of the receptor followed by the binding of the N-terminus of the chemokine. The two-step biding mechanism stabilizes the receptor in an active conformation that facilitates intracellular signaling1.

Gen-ID: 10803

NCBI Accession: NM_001256369

UniProt: P51686