CCR2 Antikörper (Extracellular, N-Term)

Produktnummer ABIN7886246

Dieser Kaninchen Polyklonal Antikörper detektiert spezifisch CCR2 in WB, FACS und LCI. Es zeigt Reaktivität gegenüber Proben von Human.

Kurzübersicht für CCR2 Antikörper (Extracellular, N-Term) (ABIN7886246)

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CCR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Flow Cytometry (FACS), Live Cell Imaging (LCI)

Produktdetails anti-CCR2 Antikörper

Bindungsspezifität: AA 11-25, Extracellular, N-Term

Verwendungszweck: A Rabbit Polyclonal Antibody to CCR2 Receptor

Kreuzreaktivität (Details): The antibody will not recognize CCR2 from mouse and rat samples.

Homologie: Human only

Produktmerkmale: Anti-Human CCR2 (extracellular) Antibody (ABIN7043032, ABIN7044170 and ABIN7044171) is a highly specific antibody directed against an epitope of the human protein. The antibody can be used in western blot, indirect live cell flow cytometry applications. It has been designed to recognize CCR2 from human samples only. The antibody will not recognize CCR2 from mouse and rat samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: RNTNESGEEVTTFFD(C), corresponding to amino acid residues 11-25 of human CCR2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:1500

FC: 2.5 μg per reaction

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu CCR2

Target: CCR2 (Chemokine (C-C Motif) Receptor 2 (CCR2))

Andere Bezeichnung: CCR2

Hintergrund:

Synonyms: C-C chemokine receptor type 2, Monocyte chemoattractant protein 1 receptor, MCP-1 receptor, CC-CKR-2, CD192

Description: CC chemokine receptor 2 (CCR2) is one of 19 members of the chemokine receptor subfamily of class A G-protein-coupled receptors (GPCRs). CCR2 is expressed in monocytes, immature dendritic cells, and T-cell subpopulations, and mediates acute inflammation by driving leukocyte migration to damaged or infected tissues towards chemokine ligands such as CCL2. Elevated expression of chemokines and their receptors such as CCR2 and its ligands can contribute to chronic inflammation and malignancy and hence these molecules are implicated in numerous inflammatory and neurodegenerative diseases including atherosclerosis, multiple sclerosis, asthma, neuropathic pain, and diabetic nephropathy, as well as cancer. These disease associations have motivated numerous studies in search of therapies that target the CCR2-chemokine axis1,2.As with most G-protein coupled receptors (GPCRs), chemokine receptors transmit signals across cell membranes by means of extracellular ligand and intracellular G-protein binding. CCR2 has seven transmembrane alpha helixes, and can take distinct conformational states that are necessary for chemokine/ligand binding, G-protein binding, activation, inactivation, and signal transmission3.CCR2 can form homo- or heterodimers with other chemokine receptors. Homodimerization may be necessary for CCR2 chemotactic activity and occurs in the absence of the ligand. CCR2/CCR5 heterocomplexes activate calcium response and support cell adhesion rather than chemotaxis, whereas CCR2/CCR4 heterodimers have an allosteric trans-inhibitory effect on CCL2 binding2.

Gen-ID: 729230

NCBI Accession: NM_000647

UniProt: P41597

Pathways: cAMP Metabolic Process, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process