HCRTR2 Antikörper (Intracellular)

Produktnummer ABIN7043029

Produktdetails anti-HCRTR2 Antikörper

Target: HCRTR2 (Hypocretin (Orexin) Receptor 2 (HCRTR2))

Bindungsspezifität: AA 391-405, Intracellular

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser HCRTR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Orexin Receptor 2

Spezifität: Intracellular, C-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Mouse - identical, human - 14,15 amino acid residues identical

Produktmerkmale: Anti-Orexin Receptor 2 Antibody (ABIN7043029, ABIN7044885 and ABIN7044886)) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunocytochemistry, and immunohistochemistry applications. It has been designed to recognize OX2R from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DRLARGRTSTESRKS, corresponding to amino acid residues 391-405 of rat OX2R

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:50

Application Dilutions Western blot wb: 1:200-1:400

Kommentare:

Cited Application: IFC

Negative Control: BLP-OR002

Blocking Peptide: BLP-OR002

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu HCRTR2

Target: HCRTR2 (Hypocretin (Orexin) Receptor 2 (HCRTR2))

Andere Bezeichnung: HCRTR2 (HCRTR2 Produkte)

Hintergrund: OX2R, Hypocretin receptor type 2, HCRTR2,Orexin Receptor 2 (OX2R) (also known as hypocretin receptor 2) is one of two receptors that recognize the peptide neurotransmitters orexin A and orexin B.1 Orexin A and B are 33 and 28 amino acids in length, respectively, and are derived from a common precursor termed prepro-orexin.OX2R binds both orexin A and B with similar affinities while OX1R binds orexin A with greater affinity than orexin B (a one order of magnitude difference).2,3Both OX2R and OX1R belong to the 7-transmembrane domain, G protein-coupled receptor (GPCR) superfamily. OX2R is thought to transmit signals through the Ga11 class of G proteins, resulting in the activation of phospholipase C with subsequent triggering of the phosphatidylinositol cascade and an influx of extracellular Ca2+, probably through transient receptor potential (TRP) channels. OX2R can also transmit signals through inhibitory Gi G proteins, although the mechanism is less well understood.2,3The physiological functions of the orexin system (OX1R, OX2R, and their ligands) have been a matter of intense research over the last few years. OX2R is expressed in both the central nervous system and peripheral locations such as gastrointestinal tissues, pancreas, and testis.2The best studied physiological role of OX2R is its involvement in the regulation of sleep and wakefulness states. Studies in mice lacking the orexin gene and dogs expressing a null mutation of the OX2R show a remarkably similar phenotype to human narcolepsy, a condition characterized by excessive daytime sleepiness, inability to maintain vigilant states, and defects in the regulation of rapid eye movement (REM) sleep.4In addition, the orexin system is involved in regulating autonomic functions such as blood pressure and heart rate, as well as in mechanisms that regulate the reward response in the brain.4

Alternative names: Orexin Receptor 2, OX2R, Hypocretin receptor type 2, HCRTR2

Gen-ID: 25605

NCBI Accession: NM_001384272

UniProt: P56719

Pathways: Feeding Behaviour