CATSPER4 Antikörper (Extracellular)

Produktnummer ABIN7043028

Dieses Anti-CATSPER4-Antikörper ist ein Kaninchen Polyklonal-Antikörper zur Detektion von CATSPER4 in WB, IF und IC. Geeignet für Maus.

Kurzübersicht für CATSPER4 Antikörper (Extracellular) (ABIN7043028)

Target: CATSPER4 (Cation Channel, Sperm Associated 4 (CATSPER4))

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CATSPER4 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunochromatography (IC)

Produktdetails anti-CATSPER4 Antikörper

Bindungsspezifität: AA 90-105, Extracellular

Verwendungszweck: A Rabbit Polyclonal Antibody to CatSper4 Channel

Spezifität: 1st extracellular loop

Kreuzreaktivität: Maus, Ratte

Homologie: Rat - identical, human -15,16 amino acid residues identical

Produktmerkmale: Anti-CatSper4 (extracellular) Antibody is directed against an epitope of mouse CatSper4. Anti-CatSper4 (extracellular) Antibody (ABIN7043028, ABIN7044103 and ABIN7044104) can be used in western blot and immunocytochemistry applications. It has been designed to recognize CatSper4 from rat, mouse and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NSYLGQKHYELFSTID, corresponding to amino acid residues 90-105 of mouse CatSper4

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:1000

Kommentare:

Cited Application: ICC

Negative Control: (ABIN7235039)

Blocking Peptide: (ABIN7235039)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CATSPER4

Target: CATSPER4 (Cation Channel, Sperm Associated 4 (CATSPER4))

Andere Bezeichnung: CATSPER4

Hintergrund: Cation channel sperm-associated protein 4,During mammalian fertilization, Ca2+ signaling plays a central role in sperm capacitation and motility to the fusion between sperm and egg1. Several Ca2+-permeable ion channel proteins have been characterized in mammalian sperm. However, only the four mammalian CatSper members (CatSpers 1-4) are restrictively expressed in the testis and are clearly shown to be required for male fertility2.CatSper channels are named after the first putative cation channel of sperm3. CatSper4 contains a single ion transport domain comprised of 6 transmembrane spanning regions, where the fourth transmembrane region resembles a voltage sensor and a pore forming region lies between transmembrane regions 5 and 6. The pore contains the consensus sequence TxDxW indicative of a probable Ca2+ selective channel4. The four CatSper proteins are primarily localized to the plasma membrane of the distal portion of the sperm tail and presumably form a heterotetrameric, pH - and voltage-dependent Ca2+-permeable channel 5. CatSper 4 is highly expressed in testis and expressed at low levels in placenta and lung6.Male mice deficient in any of the four CatSper are completely sterile but exhibit no other apparent abnormality. The major phenotypes of the null sperm are a lack of hyperactivated motility, a gradual loss of motility after isolation, and an inability to penetrate zona-intact eggs7.

Alternative names: CatSper4, Cation channel sperm-associated protein 4

Gen-ID: 329954

NCBI Accession: NM_198137

UniProt: Q8BVN3