CNR2 Antikörper (Extracellular)

Produktnummer ABIN7043026

Produktdetails anti-CNR2 Antikörper

Target: CNR2 (Cannabinoid Receptor 2 (CNR2))

Bindungsspezifität: AA 11-24, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CNR2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Cannabinoid Receptor 2

Spezifität: Extracellular, N-terminus

Kreuzreaktivität (Details): Not recommended to use with rat and mouse samples.

Homologie: Canis,bovine - 10,14 amino acid residues identical

Produktmerkmale: Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody is directed against an extracellular epitope of the human CB2 receptor. Anti-Human Cannabinoid Receptor 2 (extracellular) Antibody (ABIN7043026, ABIN7044159 and ABIN7044160)) can be used in western blot, immunohistochemistry and live cell imaging applications. It has been designed to recognize CB2 from human samples. The antibody is not recommended for use on rat and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)NGSKDGLDSNPMKD, corresponding to amino acid residues 11-24 of human CB2 receptor

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-CR003

Blocking Peptide: BLP-CR003

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CNR2

Target: CNR2 (Cannabinoid Receptor 2 (CNR2))

Andere Bezeichnung: CNR2 (CNR2 Produkte)

Hintergrund: CB2, CNR2,Cannabinoids have been used as pain relievers in Eastern medicine for many years.1 To date, two specific cannabinoid receptors have been identified: cannabinoid receptor 1 (CB1) and cannabinoid receptor 2 (CB2).2 The cannabinoid receptors can be distinguished by their amino acid sequence, signaling mechanisms and tissue distribution.2 Both receptors belong to the G-protein coupled receptor superfamily and are coupled to Gi/0 G protein.2,3The CB2 is highly expressed in cells of the immune system such as macrophages, lymphocytes natural killer cells and mast cells but has also been shown to be expressed, by both, in situ-hybridization and in immunohistochemistry, in spleen, thymus, and pancreas.1,2,4 CB2 expression in brain is still much less characterized than that of CB1. Recently, it was demonstrated that CB2 is expressed in the brain and might have a role in controlling fundamental processes such as proliferation and survival of neural cells.5,6Overexpression of CB2 was reported in several cancers such as prostate, glioma and acute myeloid leukemias.7-9 In human astrocytoma a direct relation between CB2 expression and tumor malignancy was demonstrated.8 Activation of CB2R in vivo by its agonist JWH-133, completely blocked cell growth.8 In C6 glioma, it was shown that activation of the CB2 by JWH-133 resulted in internalization of only the CB2 and not CB1 leading to apoptosis of the cells. This may well be a new approach for the treatment of glioma.

Alternative names: Cannabinoid Receptor 2, CB2, CNR2

Gen-ID: 1269

NCBI Accession: NM_001841

UniProt: P34972