CASR Antikörper (Extracellular)

Produktnummer ABIN7043018

Produktdetails anti-CASR Antikörper

Target: CASR (Calcium-Sensing Receptor (CASR))

Bindungsspezifität: AA 216-229, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser CASR Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Live Cell Imaging (LCI), Immunofluorescence (Cultured Cells) (IF (cc)), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to Calcium Sensing Receptor

Spezifität: Extracellular, N-terminus

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat,mouse,bovine,canis - identical

Produktmerkmale: Anti-Calcium Sensing Receptor (extracellular) Antibody (ABIN7043018, ABIN7044161 and ABIN7044162)) is a highly specific antibody directed against an epitope of the human CaSR. The antibody can be used in western blot, immunohistochemistry, indirect flow cytometry, and immunocytochemistry, and live cell imaging applications. It has been designed to recognize CaSR from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)DDYG RPGIE KFREE, corresponding to amino acid residues 216-229 of human CaSR

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Cited Application: IHC

Negative Control: BLP-CR004

Blocking Peptide: BLP-CR004

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu CASR

Target: CASR (Calcium-Sensing Receptor (CASR))

Andere Bezeichnung: CASR (CASR Produkte)

Hintergrund: CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A,The Calcium-sensing receptor (CaSR) is a member of subfamily C of the G-protein-coupled receptor (GPCR) superfamily. As its primary role, the CaSR is a key player in calcium homeostasis and is expressed in tissues involved in calcium metabolism such as parathyroid cells and the kidneys.1,2The expression of CaSR was also described in other cell types and tissues, such as neurons, keratinocytes, and the pancreas. However, the role of CaSR in these cells remains to be established.1,2 Following the identification of functional vitamin D response elements in the CaSR gene, it was suggested that vitamin D might regulate the expression of CaSR.3CaSR activation is followed by a rapid, transient increase in the cytosolic calcium concentration, resulting from mobilization of calcium from thapsigargin-sensitive intracellular stores, as well as an increased calcium influx through voltage-insensitive calcium channels located at the cell membrane.4CaSR regulates cellular processes such as proliferation, apoptosis, and differentiation under both normal and pathologic conditions.5Recent studies demonstrated the expression in human colon epithelium of CaSR, which regulates proliferation and differentiation. In colon carcinomas, lower levels of expression were found in the cancerous tissue compared to normal colon tissue.

Alternative names: Calcium Sensing Receptor, CaSR, Extracellular calcium-sensing receptor, Parathyroid cell calcium-sensing receptor 1, PCaR1, GPRC2A

Gen-ID: 846

NCBI Accession: NM_000388

UniProt: P41180

Pathways: Positive Regulation of Peptide Hormone Secretion, Carbohydrate Homeostasis