GPR77 Antikörper (Extracellular)

Produktnummer ABIN7042975

Produktdetails anti-GPR77 Antikörper

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Bindungsspezifität: AA 179-193, Extracellular

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser GPR77 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (Cultured Cells) (IF (cc))

Verwendungszweck: A Rabbit Polyclonal antibody to C5aR2 (extracellular).

Spezifität: Extracellular, 2nd loop.

Kreuzreaktivität: Maus, Ratte

Kreuzreaktivität (Details): Will not recognize Human C5aR2.

Homologie: Mouse - 14 out of 15 amino acid residues identical, Rat - 13 out of 15 amino acid residues identical Will not recognize Human C5aR2

Produktmerkmale: A rabbit polyclonal antibody to C5aR2 (extracellular).

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)RRLLQEHYPPRLV(S)G, corresponding to amino acid residues 179 - 193 of mouse C5aR2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:300

Application Dilutions Western blot wb: 1:400

Kommentare:

Negative Control: BLP-AR034

Blocking Peptide: BLP-AR034

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu GPR77

Target: GPR77 (G Protein-Coupled Receptor 77 (GPR77))

Andere Bezeichnung: C5AR2 (GPR77 Produkte)

Hintergrund: Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77,The complement system is a vital component of the innate immune system that play an important role in the immune responses to infectious organisms and tissue damages 1. The activation of the complement system is carried out through a proteolytic enzymes cascade generating various peptides and fragments 2.One of the most important component of the complement system is a protein named complement protein C5 3. Upon proteolytic cleavage of C5 two peptides are generated, C5a and C5b 4. C5a is known to be the most potent chemotactic factor in cells and responsible for the directed migration of cells involved in the immune response to infectious organisms and tissue damages 5. There are two different C5a receptors, C5aR1 (C5aR, CD88) and C5aR2 (C5L2, GPR77). C5aR1 is a member of the G-protein coupled receptors (GPCRs), and known to mediate the vast majority of C5a biological activity 6. In contrast C5aR2 does not exhibit any functional coupling to G proteins and it exact mechanism of action remains controversial 7. Expression of C5aR2 can be found in various tissues such as, bone marrow, spleen, lung and in immune cells such as myeloid cells and T cells 3.

Alternative names: Complement Component 5a Receptor 2, C5L2, C5a Anaphylatoxin Chemotactic Receptor 2, G Protein-Coupled Receptor 77, GPR77

Gen-ID: 319430

NCBI Accession: NM_018485

UniProt: Q8BW93