Bestrophin 2 Antikörper (3rd Extracellular Loop)

Produktnummer ABIN7886084

Der Kaninchen Polyklonal anti-Bestrophin 2 Antikörper (ABIN7886084) detektiert spezifisch Bestrophin 2 in WB und IHC. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für Bestrophin 2 Antikörper (3rd Extracellular Loop) (ABIN7886084)

Target: Bestrophin 2 (BEST2)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Bestrophin 2 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC)

Produktdetails anti-Bestrophin 2 Antikörper

Bindungsspezifität: 3rd Extracellular Loop, AA 259-270

Verwendungszweck: A Rabbit Polyclonal Antibody to Bestrophin-2 Channel

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Rat - identical, human - 11,12 amino acid residues identical

Produktmerkmale: Anti-Bestrophin-2 (extracellular) Antibody is directed against an epitope located at the 3rd extracellular loop of mouse Bestrophin-2. Anti-Bestrophin-2 (extracellular) Antibody (ABIN7042965 and ABIN7043936) can be used in western blot and immunohistochemical applications, and has been designed to recognize bestrophin-2 channel from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)DPAQGYKDHTLD, corresponding to amino acid residues 259-270 of mouse Bestrophin-2

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: 1:100

IP: The optimal concentration should be determined by the user

Kommentare:

Cited Application: IHC

Negative Control: (ABIN7234860)

Blocking Peptide: (ABIN7234860)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Bestrophin 2

Target: Bestrophin 2 (BEST2)

Andere Bezeichnung: BEST2

Hintergrund:

Synonyms: BEST2, Vitelliform macular dystrophy 2-like protein 1, VMD2L1

Description: Mammalian Cl- channels can be broadly classified into four different families: voltage-dependent Cl- channels (CLCs), the cystic fibrosis transmembrane conductance regulator (CFTR), ligand-gated Cl- channels (γ-aminobutyric acid (GABA)) and glycine channels) and Ca2+-activated Cl- channels (Bestrophin and Anoctamin channels).Bestrophins were first found by genetic linkage of human-Bestrophin-1 (hBest1) to a juvenile form of macular degeneration called Best vitelliform macular dystrophy1,2. To date Bestrophin 1-4 have been identified, although Bestrophin-3 and Bestrophin-4 have been observed only at the RNA level3. In addition, splice variants of some of these Ca2+-activated Cl- channels (CaCCs) have also been detected2,4,5. CaCCs are known to be involved in the regulation of olfaction, taste, phototransduction, and excitability in the nervous system. However, the molecular identity and functional role of CaCC in the brain have not been well established6. Bestrophin-2 is prominently expressed in colon and testes. Recently, Bestrophin-2 has also been found to be expressed in olfactory sensory neurons (OSNs)7.Two different topologies for Bestrophin-1 have been proposed. The first structure proposes that six hydrophobic domains span the membrane8, while the second suggests that there are only four membrane-spanning domains9. The same structural controversy applies to Bestrophin-2. Bestrophin-1 and Bestrophin-2 (as well as Bestrophin-4 by heterologous expression) are activated by intracellular Ca2+ 10-12. Ca2+-dependent activation of Bestrophin-2 was also demonstrated in Xenopus13.

Gen-ID: 212989

NCBI Accession: NM_017682

UniProt: Q8BGM5