ATP2A1/SERCA1 Antikörper (Cytosolic)

Produktnummer ABIN7042953

Produktdetails anti-ATP2A1/SERCA1 Antikörper

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Bindungsspezifität: AA 25-37, Cytosolic

Reaktivität: Ratte

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser ATP2A1/SERCA1 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunochromatography (IC)

Verwendungszweck: A Rabbit Polyclonal Antibody to SERCA1 Pump

Spezifität: Cytoplasmic, N-terminus

Kreuzreaktivität: Maus, Ratte

Homologie: Mouse - identical, human - 12,13 amino acid residues identical

Produktmerkmale: Anti-SERCA1 Antibody (ABIN7042953 and ABIN7044150) is a highly specific antibody directed against an epitope of the rat protein. The antibody can be used in western blot, immunohistochemistry, and immunocytochemistry applications. It has been designed to recognize SERCA1 from rat, mouse, and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: (C)TPDQVKRHLEKYG, corresponding to amino acid residues 25-37 of rat SERCA1

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: N/A

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: (ABIN7234813)

Blocking Peptide: (ABIN7234813)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: Recosntitute with double distilled water (DDW) to a concentration of 1.0 mg/mL.

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu ATP2A1/SERCA1

Target: ATP2A1/SERCA1 (ATP2A1) (ATPase, Ca++ Transporting, Cardiac Muscle, Fast Twitch 1 (ATP2A1))

Andere Bezeichnung: ATP2A1 (ATP2A1 Produkte)

Hintergrund: Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, Calcium pump 1, ATP2A1,Three Ca2+ ATPases have been described in mammalian cells. They are located in the plasma membrane, endoplasmic reticulum or the Golgi apparatus. SERCA pumps are located in both the endoplasmic reticulum and in the Golgi membranes. They are known to transport two Ca2+ molecules per hydrolysis of one ATP1. Their structure includes ten transmembrane domains and their main role is to remove cytoplasmic Ca2+ ions in order to promote muscle relaxation1.In mammals three genes encode three SERCA pumps. Each transcript undergoes tissue-dependent alternative splicing. SERCA1a and 1b are expressed in adult and neonatal skeletal muscle respectively. SERCA2a is also expressed in skeletal muscle, while SERCA2b is ubiquitously expressed. SERCA3 is expressed in a limited number of non-muscle cells2. Although all SERCAs are regulated, SERCA2b undergoes extensive regulation at the protein level, such as protein-protein interaction, phosphorylation and glycosylation1.The expression of SERCA1 in mice is essential3 whereas in human its absence is tolerated but is the cause of Brody myopathy4. Malfunction of SERCAs is also observed in heart disease and different forms of cancer1.

Alternative names: SERCA1, Sarcoplasmic/endoplasmic reticulum calcium ATPase 1, Calcium pump 1, ATP2A1

Gen-ID: 116601

NCBI Accession: NM_004320

UniProt: Q64578

Pathways: Ribonucleoside Biosynthetic Process