Anoctamin 5 Antikörper (2nd Cytoplasmic Loop)

Produktnummer ABIN7042929

Der Kaninchen Polyklonal anti-Anoctamin 5 Antikörper (ABIN7042929) detektiert spezifisch Anoctamin 5 in WB. Dieser Antikörper reagiert spezifisch mit Proben aus Maus.

Kurzübersicht für Anoctamin 5 Antikörper (2nd Cytoplasmic Loop) (ABIN7042929)

Target: Anoctamin 5 (ANO5)

Reaktivität: Maus

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser Anoctamin 5 Antikörper ist unkonjugiert

Applikation: Western Blotting (WB)

Produktdetails anti-Anoctamin 5 Antikörper

Bindungsspezifität: 2nd Cytoplasmic Loop, AA 532-544

Verwendungszweck: A Rabbit Polyclonal Antibody to Anoctamin-5 Channel

Kreuzreaktivität: Maus, Ratte

Homologie: Rat - identical, human - 12,13 amino acid residues identical

Produktmerkmale: Anti-Anoctamin-5 Antibody is directed against an epitope of mouse ANO5. Anti-Anoctamin-5 Antibody (ABIN7042929, ABIN7044135 and ABIN7044136) can be used in western blot analysis. It has been designed to recognize ANO5 from mouse, rat and human samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen: (C)TKMEIPRTHQEYE, corresponding to amino acid residues 532-544 of mouse ANO5

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

WB: 1:200

FC: The optimal concentration should be determined by the user

ICC: The optimal concentration should be determined by the user

IHC: The optimal concentration should be determined by the user

IP: The optimal concentration should be determined by the user

Kommentare:

Negative Control: (ABIN7234743)

Blocking Peptide: (ABIN7234743)

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 25 μL, 50 μL or 0.2 mL double distilled water (DDW), depending on the sample size.

Konzentration: 0.8 mg/mL

Buffer: PBS pH 7.4, 1 % BSA with 0.05 % sodium azide

Konservierungsmittel: Sodium azide

Vorsichtsmaßnahmen: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Lagerung: -20 °C

Informationen zur Lagerung: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C

Details zu Anoctamin 5

Target: Anoctamin 5 (ANO5)

Andere Bezeichnung: ANO5

Hintergrund:

Synonyms: ANO5, Transmembrane protein 16E, TMEM16E, GDD1, LGMD2L

Description: Anoctamins, also known as TMEM16, is a family of ten proteins involved in a variety of functions including phospholipid scrambling, ion transport and the regulation of other membrane proteins. ANO1 (Anoctamin-1), ANO2 and ANO5 function as Ca2+-activated Cl- channels (CaCCs) and play an important role in calcium dependent chloride secretion. All members of the Anoctamin family present high structural homology and consist of eight transmembrane domains and cytosolic N- and C-termini1.The ANO5 gene is widely distributed and expressed in bone, skeletal muscle, cardiac muscle, brain, lung and kidney and also in the mucosal layer of the GI tract and the skeletal muscle layer of the esophagus of mice2.In phylogenetic analysis ANO5 was found to be a close relative of ANO6. ANO6 has an association with phospholipid scramblase activity. Under both physiological and pathological conditions scramblase dissipates the normal asymmetry of the cell membrane by catalyzing the rapid externalization of phospholipids on the cell membrane and thus promoting apoptosis3.Recessive mutations in the ANO5 gene were found to be linked to limb-girdle muscular dystrophies type 2L (LGMD2L) and non-dysferlin Miyoshi myopathy4. LGMD2L is characterized by proximal weakness, asymmetric quadriceps femoris and atrophy of the biceps brachii muscle5.Mutations in the gene are also linked to the Gnathodiaphyseal dysplasia disease, an unusual generalized autosomal dominant disease. Patients with this syndrome show sclerosis of tubular bones, lesions of jawbones, an increased sensitivity for jaw infection and osteomyeletis and fragile bones6.

Gen-ID: 233246

NCBI Accession: NM_213599

UniProt: Q75UR0