alpha 1 Adrenergic Receptor Antikörper (Cys176Ser-Mutant, Extracellular)

Produktnummer ABIN7042908

Produktdetails anti-alpha 1 Adrenergic Receptor Antikörper

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Bindungsspezifität: AA 171-183, Cys176Ser-Mutant, Extracellular

Reaktivität: Human

Wirt: Kaninchen

Klonalität: Polyklonal

Konjugat: Dieser alpha 1 Adrenergic Receptor Antikörper ist unkonjugiert

Applikation: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunofluorescence (Cultured Cells) (IF (cc)), Live Cell Imaging (LCI)

Verwendungszweck: A Rabbit Polyclonal Antibody to ?1A-Adrenergic Receptor

Spezifität: 2nd extracellular loop

Kreuzreaktivität: Human, Maus, Ratte

Homologie: Human,rat,mouse,bovine - 12,13 amino acid residues identical

Produktmerkmale: Anti-alpha1A-Adrenergic Receptor (extracellular) Antibody is directed against an extracellular epitope of the human α1A-adrenergic receptor. Anti-α1A-Adrenergic Receptor (extracellular) Antibody (ABIN7042908, ABIN7043908 and ABIN7043909)) can be used in western blot and immunohistochemistry applications. It has been designed to recognize α1A-adrenoceptor from human, rat, and mouse samples.

Aufreinigung: Affinity purified on immobilized antigen.

Immunogen:

Immunogen: Synthetic peptide

Immunogen Sequence: EDETI*SQINEEPG(C), corresponding to amino acid residues 171-183 of human alpha1A-adrenoceptor with replacement of cysteine 176 (C176) with serine (*S) 

Isotyp: IgG

Anwendungsinformationen

Applikationshinweise:

Antigen preadsorption control: 1 μg peptide per 1 μg antibody

Application Dilutions Immunohistochemistry paraffin embedded sections ihc: 1:100

Application Dilutions Western blot wb: 1:200

Kommentare:

Negative Control: BLP-AR015

Blocking Peptide: BLP-AR015

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Format: Lyophilized

Rekonstitution: 0.2 mL double distilled water (DDW).

Konzentration: 1 mg/mL

Buffer: PBS pH 7.4

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung:

Storage before reconstitution: The antibody ships as a lyophilized powder at room temperature. Upon arrival, it should be stored at -20°C.

Storage after reconstitution: The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods, small aliquots should be stored at -20°C. Avoid multiple freezing and thawing. Centrifuge all antibody preparations before use (10000 x g 5 min).

Details zu alpha 1 Adrenergic Receptor

Target: alpha 1 Adrenergic Receptor (ADRA1A) (Adrenoceptor alpha 1A (ADRA1A))

Andere Bezeichnung: ADRA1A (ADRA1A Produkte)

Hintergrund: Alpha-1A adrenoceptor, Alpha-1C adrenergic receptor, α1A-AR, ADRA1A,Adrenergic receptors (also called adrenoceptors) are the receptors for the catecholamines adrenaline and noradrenaline (called epinephrine and norepinephrine in the United States). Adrenaline and noradrenaline play important roles in the control of blood pressure, myocardial contractile rate and force, airway reactivity, and a variety of metabolic and central nervous system functions.Adrenergic receptors are members of the G-protein coupled receptor (GPCR) superfamily of membrane proteins. They share a common structure of seven putative transmembrane domains, an extracellular amino terminus, and a cytoplasmic carboxyl terminus.Adrenoceptors are divided into three types: α1, α2 and β adrenoceptors. Each type is further divided into at least three subtypes: α1A, α1B, α1D, α2A, α2B, α2C, β1, β2, β3.1,2 They are expressed in nearly all peripheral tissues and in the central nervous system.1,2All α1-adrenoceptors (α1-ARs) activate phospholipases C and A2.3 In addition to mobilizing intracellular calcium, the α1-ARs have also been shown to activate calcium influx via voltage-dependent and -independent calcium channels.4The α1A-ARs have at least nine known alternative spliced isoforms. They are the most abundant adrenergic receptor in the brain, and are considered postsynaptic receptors. α1A-ARs are also expressed in the heart, liver and prostate.

Alternative names: alpha1A-Adrenergic Receptor, Alpha-1A adrenoceptor, Alpha-1C adrenergic receptor, alpha1A-AR, ADRA1A

Gen-ID: 148

NCBI Accession: NM_033303

UniProt: P35348

Pathways: AMPK Signaling